ATTENTION: The software behind KU ScholarWorks is being upgraded to a new version. Starting July 15th, users will not be able to log in to the system, add items, nor make any changes until the new version is in place at the end of July. Searching for articles and opening files will continue to work while the system is being updated.
If you have any questions, please contact Marianne Reed at mreed@ku.edu .
Molecular Biosciences: Recent submissions
Now showing items 541-560 of 887
-
Revisiting Hydrophobic Mismatch with Free Energy Simulation Studies of Transmembrane Helix Tilt and Rotation
(Elsevier, 2010-07)Protein-lipid interaction and bilayer regulation of membrane protein functions are largely controlled by the hydrophobic match between the transmembrane (TM) domain of membrane proteins and the surrounding lipid bilayer. ... -
A Repulsive Electrostatic Mechanism for Protein Export through the Type III Secretion Apparatus
(Elsevier, 2010-02)Many Gram-negative bacteria initiate infections by injecting effector proteins into host cells through the type III secretion apparatus, which is comprised of a basal body, a needle, and a tip. The needle channel is formed ... -
Comparative Molecular Dynamics Simulation Studies of Protegrin-1 Monomer and Dimer in Two Different Lipid Bilayers
(2009-08)Antimicrobial peptides interact specifically with the membrane of a pathogen and kill the pathogen by releasing its cellular contents. Protegrin-1 (PG-1), a β-hairpin antimicrobial peptide, is known to exist as a transmembrane ... -
Alanine-scanning mutagenesis of Aspergillus γ-tubulin yields diverse and novel phenotypes
(American Society for Cell Biology, 2001-07-01)We have created 41 clustered charged-to-alanine scanning mutations of the mipA, γ-tubulin, gene of Aspergillus nidulans and have created strains carrying these mutations by two-step gene replacement and by a new procedure, ... -
CHARMM-GUI Membrane Builder for Mixed Bilayers and Its Application to Yeast Membranes
(Elsevier, 2009-02)Most biological membranes are composed of many different kinds of lipid, and can be characterized by the composition of the lipids. Although more and more researchers have shown their interests in molecular dynamics ... -
A mutation in γ-tubulin alters microtubule dynamics and organization and is synthetically lethal with the kinesin-like protein Pkl1p
(American Society for Cell Biology, 2000-04-01)Mitotic segregation of chromosomes requires spindle pole functions for microtubule nucleation, minus end organization, and regulation of dynamics. γ-Tubulin is essential for nucleation, and we now extend its role to these ... -
Membrane Assembly of Simple Helix Homo-Oligomers Studied via Molecular Dynamics Simulations
(Elsevier, 2007-02)The assembly of simple transmembrane helix homo-oligomers is studied by combining a generalized Born implicit membrane model with replica exchange molecular dynamics simulations to sample the conformational space of various ... -
The spindle pole body of Schizosaccharomyces pombe enters and leaves the nuclear envelope as the cell cycle proceeds
(American Society for Cell Biology, 1997-08-01)he cycle of spindle pole body (SPB) duplication, differentiation, and segregation in Schizosaccharomyces pombe is different from that in some other yeasts. Like the centrosome of vertebrate cells, the SPB of S. pombe spends ... -
Human gamma-tubulin functions in fission yeast
(Rockefeller University Press, 1994-09-15)gamma-Tubulin is a phylogenetically conserved component of microtubule-organizing centers that is essential for viability and microtubule function. To examine the functional conservation of gamma-tubulin, we have tested ... -
Mitochondria and nuclei move by different mechanisms in Aspergillus nidulans
(Rockefeller University Press, 1985-12-01)We have examined the effects of the antimicrotubule agent benomyl and several mutations on nuclear and mitochondrial movement in germlings of the filamentous fungus Aspergillus nidulans. While, as previously reported, ... -
A mutation in Aspergillus nidulans that blocks the transition from interphase to prophase
(Rockefeller University Press, 1983-04-01)In order to develop a method for obtaining mitotic synchrony in aspergillus nidulans, we have characterized previously isolated heat-sensitive nim mutations that block the nuclear division cycle in interphase at restrictive ... -
Kinetochores associated with the nuclear envelope in the mitosis of a dinoflagellate
(Rockefeller University Press, 1974-10-01)No abstract available -
APC1638T: a mouse model delineating critical domains of the adenomatous polyposis coli protein involved in tumorigenesis and development
(Cold Spring Harbor Laboratory Press, 1999-04-01)The adenomatous polyposis coli (APC) gene is considered as the true gatekeeper of colonic epithelial proliferation: It is mutated in the majority of colorectal tumors, and mutations occur at early stages of tumor development ... -
Ion Permeation through the α-Hemolysin Channel: Theoretical Studies Based on Brownian Dynamics and Poisson-Nernst-Plank Electrodiffusion Theory
(Elsevier, 2004-10)Identification of the molecular interaction governing ion conduction through biological pores is one of the most important goals of modern electrophysiology. Grand canonical Monte Carlo Brownian dynamics (GCMC/BD) and ... -
An Implicit Membrane Generalized Born Theory for the Study of Structure, Stability, and Interactions of Membrane Proteins
(Elsevier, 2003-11)Exploiting recent developments in generalized Born (GB) electrostatics theory, we have reformulated the calculation of the self-electrostatic solvation energy to account for the influence of biological membranes. Consistent ... -
Imaging the Electrostatic Potential of Transmembrane Channels: Atomic Probe Microscopy of OmpF Porin
(Elsevier, 2002-03)The atomic force microscope (AFM) was used to image native OmpF porin and to detect the electrostatic potential generated by the protein. To this end the OmpF porin trimers from Escherichia coli was reproducibly imaged at ... -
A Grand Canonical Monte Carlo–Brownian Dynamics Algorithm for Simulating Ion Channels
(Elsevier B. V., 2000-08)A computational algorithm based on Grand Canonical Monte Carlo (GCMC) and Brownian Dynamics (BD) is described to simulate the movement of ions in membrane channels. The proposed algorithm, GCMC/BD, allows the simulation ... -
Simple Resazurin-Based Microplate Assay for Measuring Chlamydia Infections
(American Society for Microbiology, 2013-06)The conventional method for quantification of Chlamydia infection using fluorescence microscopy typically involves time- and labor-intensive manual enumeration, which is not applicable for a large-scale analysis required ... -
Expression, purification, crystallization and preliminary X-ray analysis of the DNA-binding domain of a Chlamydia trachomatis OmpR/PhoB-subfamily response regulator homolog, ChxR
(International Union of Crystallography, 2009-08)Two-component signal transduction systems in bacteria are a primary mechanism for responding to environmental stimuli and adjusting gene expression accordingly. Generally in these systems a sensor kinase phosphorylates a ... -
Global Analysis of Borrelia burgdorferi Genes Regulated by Mammalian Host-Specific Signals
(American Society for Microbiology, 2003-06)Lyme disease is a tick-borne infection that can lead to chronic, debilitating problems if not recognized or treated appropriately. Borrelia burgdorferi, the causative agent of Lyme disease, is maintained in nature by a ...