Molecular Biosciences
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Natural product (L)-gossypol inhibits colon cancer cell growth by targeting RNA-binding protein Musashi-1
(Wiley Open Access, 2015-04-10)Musashi-1 (MSI1) is an RNA-binding protein that acts as a translation activator or repressor of target mRNAs. The best-characterized MSI1 target is Numb mRNA, whose encoded protein negatively regulates Notch signaling. ... -
Overexpression of the Aspergillus nidulans histone 4 acetyltransferase EsaA increases activation of secondary metabolite production
(Wiley, 2012-10)Regulation of secondary metabolite (SM) gene clusters in Aspergillus nidulans has been shown to occur through cluster specific transcription factors or through global regulators of chromatin structure such as histone ... -
Chlamydia trachomatis protein CT009 is a structural and functional homolog to the key morphogenesis component RodZ and interacts with division septal plane localized MreB
(Wiley, 2015-02)Cell division in Chlamydiae is poorly understood as apparent homologs to most conserved bacterial cell division proteins are lacking and presence of elongation (rod shape) associated proteins indicate non-canonical mechanisms ... -
A de novo protein binding pair by computational design and directed evolution
(Elsevier, 2011-04-22)The de novo design of protein-protein interfaces is a stringent test of our understanding of the principles underlying protein-protein interactions and would enable new approaches to biological and medical challenges. Here ... -
Molecular genetic analysis of the orsellinic acid/F9775 gene cluster of Aspergillus nidulans†,‡
(Royal Society of Chemistry, 2010-03)F-9775A and F-9775B are cathepsin K inhibitors that arise from a chromatin remodelling deletant strain of Aspergillus nidulans. A polyketide synthase gene has been determined to be responsible for their formation and for ... -
Identification and molecular genetic analysis of the cichorine gene cluster in Aspergillus nidulans
(Royal Society of Chemistry, 2012-08-01)We recently demonstrated that the phytotoxin cichorine is produced by Aspergillus nidulans. Through a set of targeted deletions, we have found a cluster of seven genes that are required for its biosynthesis. Two of the ... -
Sec61á is required for dorsal closure during Drosophila embryogenesis through its regulation of Dpp signaling
(Wiley, 2010-03)During dorsal closure in Drosophila, signaling events in the dorsalmost row of epidermal cells (DME cells) direct the migration of lateral epidermal sheets towards the dorsal midline where they fuse to enclose the embryo. ... -
A Flow Cytometric Assay for the Study of E3 Ubiquitin Ligase Activityb
(Wiley, 2009-07)BACKGROUND: Current methods for monitoring E3 ubiquitin ligase activity in cell culture or in vivo are limited. As a result, the degradation of cellular targets by many E3 ubiquitin ligases in live cells has not yet been ... -
Loss of dystrophin and the microtubule-binding protein ELP-1 causes progressive paralysis and death of adult C. elegans
(Wiley, 2009-08)EMAP-like proteins (ELPs) are conserved microtubule-binding proteins that function during cell division and in the behavior of post-mitotic cells. In C. elegans, ELP-1 is broadly expressed in many cells and tissues including ... -
Protein Structure Prediction: Is It Useful?
(Elsevier, 2009-04)Computationally predicted three-dimensional structure of protein molecules has demonstrated the usefulness in many areas of biomedicine, ranging from approximate family assignments to precise drug screening. For nearly 40 ... -
Low-resolution structural modeling of protein interactome
(Elsevier, 2013-04)Structural characterization of protein–protein interactions across the broad spectrum of scales is key to our understanding of life at the molecular level. Low-resolution approach to protein interactions is needed for ... -
Computational Design of Affinity and Specificity at Protein-Protein Interfaces
(Elsevier, 2009-08)The computer-based design of protein-protein interactions is a rigorous test of our understanding of molecular recognition and an attractive approach for creating novel tools for cell and molecular research. Considerable ... -
Elimination of T cell reactivity to pancreatic β cells and partial preservation of β cell activity by peptide blockade of LFA-1:ICAM-1 interaction in the NOD mouse model
(Elsevier, 2013-08)In insulin dependent diabetes mellitus (T1D), self-reactive T cells infiltrate pancreatic islets and induce beta cell destruction and dysregulation of blood glucose. A goal is to control only the self-reactive T cells, ... -
The designability of protein switches by chemical rescue of structure: mechanisms of inactivation and reactivation
(American Chemical Society, 2013-12-18)The ability to selectively activate function of particular proteins via pharmacological agents is a longstanding goal in chemical biology. Recently, we reported an approach for designing a de novo allosteric effector site ... -
Novel Pyrrolopyrimidine-Based α-Helix Mimetics: Cell- Permeable Inhibitors of Protein-Protein Interactions
(American Chemical Society, 2011-02-02)There is considerable interest in developing nonpeptidic, small molecule α-helix mimetics to disrupt α-helix-mediated protein-protein interactions. Herein, we report the design of a novel pyrrolopyrimidine-based scaffold ... -
Illuminating the diversity of aromantic polyketide synthases in Aspergillus nidulans
(American Chemical Society, 2012-05-16)Genome sequencing has revealed that fungi have the ability to synthesize many more natural products (NPs) than are currently known, but methods for obtaining suitable expression of NPs have been inadequate. We have developed ... -
Genome-Based Deletion Analysis Reveals the Prenyl Xanthone Biosynthesis Pathway in Aspergillus nidulans
(American Chemical Society, 2011-03-23)Xanthones are a class of molecules that bind to a number of drug targets and possess a myriad of biological properties. An understanding of xanthone biosynthesis at the genetic level should facilitate engineering of ... -
Entropic and enthalpic components of catalysis in the mutase and lyase activities of Pseudomonas aeruginosa PchB
(American Chemical Society, 2011-05-11)The isochorismate-pyruvate lyase from Pseudomonas aeruginosa (PchB) catalyzes two pericyclic reactions, demonstrating the eponymous activity and also chorismate mutase activity. The thermodynamic parameters for these ... -
An Efficient System for Heterologous Expression of Secondary Metabolite Genes in Aspergillus nidulans
(American Chemical Society, 2013-05-22)Fungal secondary metabolites (SMs) are an important source of medically valuable compounds. Genome projects have revealed that fungi have many SM biosynthetic gene clusters that are not normally expressed. To access these ... -
3-Substituted biquinolinium inhibitors of AraC family transcriptional activator VirF from S. flexneri obtained through in situ chemical ionization of 3,4-disubstituted dihydroquinolines
(Royal Society of Chemistry, 2014-08-18)During a structure–activity relationship optimization campaign to develop an inhibitor of AraC family transcriptional activators, we discovered an unexpected transformation of a previously reported inhibitor that occurs ...
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