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    A mutation in Aspergillus nidulans that blocks the transition from interphase to prophase

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    Oakley_JCB_96(4)1155.pdf (1.248Mb)
    Issue Date
    1983-04-01
    Author
    Oakley, Berl R.
    Morris, N. Ronald
    Publisher
    Rockefeller University Press
    Type
    Article
    Article Version
    Scholarly/refereed, publisher version
    Metadata
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    Abstract
    In order to develop a method for obtaining mitotic synchrony in aspergillus nidulans, we have characterized previously isolated heat-sensitive nim mutations that block the nuclear division cycle in interphase at restrictive temperature. After 3.5 h at restrictive temperature the mitotic index of a strain carrying one of these mutations, nimA5, was 0, but when this strain was subsequently shifted from restrictive to permissive temperature the mitotic index increased rapidly, reaching a maximum of 78 percent after 7.5 min. When this strain was examined electron-microscopically, mitotic spindles were absent at restrictive temperature. From these data we conclude that at restrictive temperature nimA5 blocks the nuclear division cycle at a point immediately preceding the initiation of chromosomal condensation and mitotic microtubule assembly, and upon shifting to permissive control over the initiation of microtubule assembly and chromosomal condensation in vivo through a simple temperature shift and, consequently, nimA5 should be a powerful tool for studying these processes. Electron-microscopic examination of spindles of material synchronized in this manner reveals that spindle formation, although very rapid, is gradual in the sense that spindle microtubule numbers increase as spindle formation proceeds.
    Description
    This is the publisher's version, also available electronically from "http://jcb.rupress.org".
    URI
    http://hdl.handle.net/1808/17454
    DOI
    https://doi.org/10.1083/jcb.96.4.1155
    ISSN
    0021-9525
    Collections
    • Molecular Biosciences Scholarly Works [606]
    Citation
    Oakley, B. (1983). A mutation in aspergillus nidulans that blocks the transition from interphase to prophase. The Journal of Cell Biology, 96(4), 1155-1158. http://www.dx.doi.org/10.1083/jcb.96.4.1155

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    Contact KU ScholarWorks
    785-864-8983
    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    785-864-8983

    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    Image Credits
     

     

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