The conventional method for quantification of Chlamydia infection using fluorescence microscopy typically involves time- and labor-intensive manual enumeration, which is not applicable for a large-scale analysis required for an inhibitory compound screen. In this study, an alamarBlue (resazurin) assay was adopted to measure Chlamydia infection by measuring the redox capability of infected host cells in a 96-well format. The assay provided measurements comparable to those of the conventional microscopy method while drastically reducing the time required for analysis.
This is the publisher's version. Copyright 2013 by the American Society for Microbiology.
Osaka, I., and P. S. Hefty. "Simple Resazurin-Based Microplate Assay for Measuring Chlamydia Infections." Antimicrobial Agents and Chemotherapy 57.6 (2013): 2838-840. http://dx.doi.org/10.1128/AAC.00056-13.
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