Simple Resazurin-Based Microplate Assay for Measuring Chlamydia Infections
Issue Date
2013-06Author
Osaka, Ichie
Hefty, P. Scott
Publisher
American Society for Microbiology
Type
Article
Article Version
Scholarly/refereed, publisher version
Metadata
Show full item recordAbstract
The conventional method for quantification of Chlamydia infection using fluorescence microscopy typically involves time- and labor-intensive manual enumeration, which is not applicable for a large-scale analysis required for an inhibitory compound screen. In this study, an alamarBlue (resazurin) assay was adopted to measure Chlamydia infection by measuring the redox capability of infected host cells in a 96-well format. The assay provided measurements comparable to those of the conventional microscopy method while drastically reducing the time required for analysis.
Description
This is the publisher's version. Copyright 2013 by the American Society for Microbiology.
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Citation
Osaka, I., and P. S. Hefty. "Simple Resazurin-Based Microplate Assay for Measuring Chlamydia Infections." Antimicrobial Agents and Chemotherapy 57.6 (2013): 2838-840. http://dx.doi.org/10.1128/AAC.00056-13.
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