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dc.contributor.authorSung, Calvin T.
dc.contributor.authorChang, Shu-Lin
dc.contributor.authorEntwistle, Ruth
dc.contributor.authorAhn, Green
dc.contributor.authorLin, Tzu-Shyang
dc.contributor.authorPetrova, Vessela
dc.contributor.authorYeh, Hsu-Hua
dc.contributor.authorPraseuth, Mike B.
dc.contributor.authorChiang, Yi-Ming
dc.contributor.authorOakley, Berl R.
dc.contributor.authorWang, Clay C. C.
dc.identifier.citationSung, C. T., Chang, S. L., Entwistle, R., Ahn, G., Lin, T. S., Petrova, V., … Wang, C. (2017). Overexpression of a three-gene conidial pigment biosynthetic pathway in Aspergillus nidulans reveals the first NRPS known to acetylate tryptophan. Fungal genetics and biology : FG & B, 101, 1–6. doi:10.1016/j.fgb.2017.01.006en_US
dc.descriptionThis work is licensed under a Creative Commons Attribution Non-Commercial-No Derivatives 4.0 International License.en_US
dc.description.abstractFungal nonribosomal peptide synthetases (NRPSs) are megasynthetases that produce cyclic and acyclic peptides. In Aspergillus nidulans, the NRPS ivoA (AN10576) has been associated with the biosynthesis of grey-brown conidiophore pigments. Another gene, ivoB (AN0231), has been demonstrated to be an N-acetyl-6-hydroxytryptophan oxidase that putatively acts downstream of IvoA. A third gene, ivoC, has also been predicted to be involved in pigment biosynthesis based on publicly available genomic and transcriptomic information. In this paper, we report the replacement of the promoters of the ivoA, ivoB, and ivoC genes with the inducible promoter alcA in a single cotransformation. Co-overexpression of the three genes resulted in the production of a dark-brown pigment in hyphae. In addition, overexpression of each of the Ivo genes, ivoA-C, individually or in combination, allowed us to isolate intermediates and confirm the function of each gene. IvoA was found to be the first known NRPS to carry out the acetylation of the amino acid, tryptophan.en_US
dc.rights© 2017 Elsevier Inc. All rights reserved.en_US
dc.subjectAspergillus nidulansen_US
dc.subjectNonribosomal peptide synthetase (NRPS)en_US
dc.subjectPigment biosynthesisen_US
dc.subjectTryptophan N-acetyltransferaseen_US
dc.subjectN-acetyltryptophan oxidaseen_US
dc.titleOverexpression of a three-gene conidial pigment biosynthetic pathway in Aspergillus nidulans reveals the first NRPS known to acetylate tryptophanen_US
kusw.kuauthorEntwistle, Ruth
kusw.kuauthorOakley, Berl R.
kusw.kudepartmentDepartment of Molecular Biosciencesen_US
kusw.oaversionScholarly/refereed, author accepted manuscripten_US
kusw.oapolicyThis item meets KU Open Access policy criteria.en_US

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© 2017 Elsevier Inc. All rights reserved.
Except where otherwise noted, this item's license is described as: © 2017 Elsevier Inc. All rights reserved.