Overexpression of a three-gene conidial pigment biosynthetic pathway in Aspergillus nidulans reveals the first NRPS known to acetylate tryptophan

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Issue Date
2017-01-17Author
Sung, Calvin T.
Chang, Shu-Lin
Entwistle, Ruth
Ahn, Green
Lin, Tzu-Shyang
Petrova, Vessela
Yeh, Hsu-Hua
Praseuth, Mike B.
Chiang, Yi-Ming
Oakley, Berl R.
Wang, Clay C. C.
Publisher
Elsevier
Type
Article
Article Version
Scholarly/refereed, author accepted manuscript
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© 2017 Elsevier Inc. All rights reserved.
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Fungal nonribosomal peptide synthetases (NRPSs) are megasynthetases that produce cyclic and acyclic peptides. In Aspergillus nidulans, the NRPS ivoA (AN10576) has been associated with the biosynthesis of grey-brown conidiophore pigments. Another gene, ivoB (AN0231), has been demonstrated to be an N-acetyl-6-hydroxytryptophan oxidase that putatively acts downstream of IvoA. A third gene, ivoC, has also been predicted to be involved in pigment biosynthesis based on publicly available genomic and transcriptomic information. In this paper, we report the replacement of the promoters of the ivoA, ivoB, and ivoC genes with the inducible promoter alcA in a single cotransformation. Co-overexpression of the three genes resulted in the production of a dark-brown pigment in hyphae. In addition, overexpression of each of the Ivo genes, ivoA-C, individually or in combination, allowed us to isolate intermediates and confirm the function of each gene. IvoA was found to be the first known NRPS to carry out the acetylation of the amino acid, tryptophan.
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This work is licensed under a Creative Commons Attribution Non-Commercial-No Derivatives 4.0 International License.
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Sung, C. T., Chang, S. L., Entwistle, R., Ahn, G., Lin, T. S., Petrova, V., … Wang, C. (2017). Overexpression of a three-gene conidial pigment biosynthetic pathway in Aspergillus nidulans reveals the first NRPS known to acetylate tryptophan. Fungal genetics and biology : FG & B, 101, 1–6. doi:10.1016/j.fgb.2017.01.006
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