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dc.contributor.authorBhattarai, Apurba
dc.contributor.authorWang, Jinan
dc.contributor.authorMiao, Yinglong
dc.date.accessioned2020-03-27T19:01:03Z
dc.date.available2020-03-27T19:01:03Z
dc.date.issued2019-10-10
dc.identifier.citationBhattarai, A., Wang, J., & Miao, Y. (2020). G-Protein-Coupled Receptor-Membrane Interactions Depend on the Receptor Activation State. Journal of computational chemistry, 41(5), 460–471. https://doi.org/10.1002/jcc.26082en_US
dc.identifier.urihttp://hdl.handle.net/1808/30196
dc.descriptionThis is the peer reviewed version of the following article: Bhattarai, A., Wang, J., & Miao, Y. (2020). G-Protein-Coupled Receptor-Membrane Interactions Depend on the Receptor Activation State. Journal of computational chemistry, 41(5), 460–471. https://doi.org/10.1002/jcc.26082, which has been published in final form at https://doi.org/10.1002/jcc.26082. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.en_US
dc.description.abstractG‐protein‐coupled receptors (GPCRs) are the largest family of human membrane proteins and serve as primary targets of approximately one‐third of currently marketed drugs. In particular, adenosine A1 receptor (A1AR) is an important therapeutic target for treating cardiac ischemia–reperfusion injuries, neuropathic pain, and renal diseases. As a prototypical GPCR, the A1AR is located within a phospholipid membrane bilayer and transmits cellular signals by changing between different conformational states. It is important to elucidate the lipid–protein interactions in order to understand the functional mechanism of GPCRs. Here, all‐atom simulations using a robust Gaussian accelerated molecular dynamics (GaMD) method were performed on both the inactive (antagonist bound) and active (agonist and G‐protein bound) A1AR, which was embedded in a 1‐palmitoyl‐2‐oleoyl‐glycero‐3‐phosphocholine (POPC) lipid bilayer. In the GaMD simulations, the membrane lipids played a key role in stabilizing different conformational states of the A1AR. Our simulations further identified important regions of the receptor that interacted distinctly with the lipids in highly correlated manner. Activation of the A1AR led to differential dynamics in the upper and lower leaflets of the lipid bilayer. In summary, GaMD enhanced simulations have revealed strongly coupled dynamics of the GPCR and lipids that depend on the receptor activation state.en_US
dc.publisherWileyen_US
dc.rights© 2019 Wiley Periodicals, Inc.en_US
dc.subjectG-protein-coupled receptorsen_US
dc.subjectAdenosine A1 receptoren_US
dc.subjectEnhanced samplingen_US
dc.subjectGaussian accelerated molecular dynamicsen_US
dc.subjectProtein-lipid interactionsen_US
dc.titleG‐Protein‐Coupled Receptor–Membrane Interactions Depend on the Receptor Activation Stateen_US
dc.typeArticleen_US
kusw.kuauthorBhattarai, Apurba
kusw.kuauthorWang, Jinan
kusw.kuauthorMiao, Yinglong
kusw.kudepartmentMolecular Biosciencesen_US
dc.identifier.doi10.1002/jcc.26082en_US
kusw.oaversionScholarly/refereed, author accepted manuscripten_US
kusw.oapolicyThis item meets KU Open Access policy criteria.en_US
dc.identifier.pmidPMC7026935en_US
dc.rights.accessrightsopenAccessen_US


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