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    G‐Protein‐Coupled Receptor–Membrane Interactions Depend on the Receptor Activation State

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    Bhattarai_2019.pdf (1.704Mb)
    Issue Date
    2019-10-10
    Author
    Bhattarai, Apurba
    Wang, Jinan
    Miao, Yinglong
    Publisher
    Wiley
    Type
    Article
    Article Version
    Scholarly/refereed, author accepted manuscript
    Rights
    © 2019 Wiley Periodicals, Inc.
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    Abstract
    G‐protein‐coupled receptors (GPCRs) are the largest family of human membrane proteins and serve as primary targets of approximately one‐third of currently marketed drugs. In particular, adenosine A1 receptor (A1AR) is an important therapeutic target for treating cardiac ischemia–reperfusion injuries, neuropathic pain, and renal diseases. As a prototypical GPCR, the A1AR is located within a phospholipid membrane bilayer and transmits cellular signals by changing between different conformational states. It is important to elucidate the lipid–protein interactions in order to understand the functional mechanism of GPCRs. Here, all‐atom simulations using a robust Gaussian accelerated molecular dynamics (GaMD) method were performed on both the inactive (antagonist bound) and active (agonist and G‐protein bound) A1AR, which was embedded in a 1‐palmitoyl‐2‐oleoyl‐glycero‐3‐phosphocholine (POPC) lipid bilayer. In the GaMD simulations, the membrane lipids played a key role in stabilizing different conformational states of the A1AR. Our simulations further identified important regions of the receptor that interacted distinctly with the lipids in highly correlated manner. Activation of the A1AR led to differential dynamics in the upper and lower leaflets of the lipid bilayer. In summary, GaMD enhanced simulations have revealed strongly coupled dynamics of the GPCR and lipids that depend on the receptor activation state.
    Description
    This is the peer reviewed version of the following article: Bhattarai, A., Wang, J., & Miao, Y. (2020). G-Protein-Coupled Receptor-Membrane Interactions Depend on the Receptor Activation State. Journal of computational chemistry, 41(5), 460–471. https://doi.org/10.1002/jcc.26082, which has been published in final form at https://doi.org/10.1002/jcc.26082. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
    URI
    http://hdl.handle.net/1808/30196
    DOI
    https://doi.org/10.1002/jcc.26082
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    • Molecular Biosciences Scholarly Works [581]
    Citation
    Bhattarai, A., Wang, J., & Miao, Y. (2020). G-Protein-Coupled Receptor-Membrane Interactions Depend on the Receptor Activation State. Journal of computational chemistry, 41(5), 460–471. https://doi.org/10.1002/jcc.26082

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    Contact KU ScholarWorks
    785-864-8983
    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    785-864-8983

    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    Image Credits
     

     

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