Efflux Pumps Represent Possible Evolutionary Convergence onto the Beta Barrel Fold
Issue Date
2018-02-19Author
Franklin, Meghan Whitney
Nepomnyachiy, Sergey
Feehan, Ryan
Ben-Tal, Nir
Kolodny, Rachel
Slusky, Joanna S.G.
Publisher
bioRxiv
Type
Preprint
Rights
Copyright 2018, The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
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Show full item recordAbstract
There are around 100 types of integral outer membrane proteins in each Gram negative bacteria. All of these proteins have the same fold—an up-down β-barrel. It has been suggested that all membrane β-barrels other than lysins are homologous. Here we suggest that β-barrels of efflux pumps have converged on this fold as well. By grouping structurally-solved outer membrane β-barrels (OMBBs) by sequence we find evidence that the membrane environment may have led to convergent evolution of the barrel fold. Specifically, the lack of sequence linkage to other barrels coupled with distinctive structural differences, such as differences in strand tilt and barrel radius, suggest that efflux pumps have evolutionarily converged on the barrel. Finally, we find a possible ancestor for the OMBB efflux pumps as they are related to periplasmic components of the same pumps.
Description
Preprint now published in Structure doi: 10.1016/j.str.2018.06.007
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