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    Molecular genetic analysis of the orsellinic acid/F9775 gene cluster of Aspergillus nidulans†,‡

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    Oakley_2010.pdf (487.2Kb)
    Issue Date
    2010-03
    Author
    Sanchez, James F.
    Chiang, Yi-Ming
    Szewczyk, Edyta
    Davidson, Ashley D.
    Ahuja, Manmeet
    Oakley, C. Elizabeth
    Bok, Jin Woo
    Keller, Nancy P.
    Oakley, Berl R.
    Wang, Clay C. C.
    Publisher
    Royal Society of Chemistry
    Type
    Article
    Article Version
    Scholarly/refereed, author accepted manuscript
    Metadata
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    Abstract
    F-9775A and F-9775B are cathepsin K inhibitors that arise from a chromatin remodelling deletant strain of Aspergillus nidulans. A polyketide synthase gene has been determined to be responsible for their formation and for the simpler, archetypical polyketide orsellinic acid. We have discovered simple culture conditions that result in the production of the three compounds, and this facilitates analysis of the genes responsible for their synthesis. We have now analysed the F9775/orsellinic acid gene cluster using a set of targeted deletions. We find that the polyketide synthase alone is required for orsellinic acid biosynthesis and only two additional genes in the cluster are required for F9775 A and B synthesis. Our deletions also yielded the bioactive metabolites gerfelin and diorcinol.
    URI
    http://hdl.handle.net/1808/24582
    DOI
    https://doi.org/10.1039/b904541d.
    Collections
    • Molecular Biosciences Scholarly Works [537]
    Citation
    Sanchez, J. F., Chiang, Y.-M., Szewczyk, E., Davidson, A. D., Ahuja, M., Oakley, C. E., … Wang, C. C. C. (2010). Molecular genetic analysis of the orsellinic acid/F9775 gene cluster of Aspergillus nidulans. Molecular bioSystems, 6(3), 587–593. http://doi.org/10.1039/b904541d

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    Contact KU ScholarWorks
    785-864-8983
    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    785-864-8983

    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    Image Credits
     

     

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