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dc.contributor.authorYeh, Hsu-Hua
dc.contributor.authorChiang, Yi-Ming
dc.contributor.authorEntwistle, Ruth A.
dc.contributor.authorAhuja, Manmeet
dc.contributor.authorLee, Kuan-Han
dc.contributor.authorBruno, Kenneth S.
dc.contributor.authorWu, Tung-Kung
dc.contributor.authorOakley, Berl R.
dc.contributor.authorWang, Clay C. C.
dc.date.accessioned2017-04-06T21:05:47Z
dc.date.available2017-04-06T21:05:47Z
dc.date.issued2012-11
dc.identifier.citationYeh, HH., Chiang, YM., Entwistle, R. et al. Appl Microbiol Biotechnol (2012) 96: 739. doi:10.1007/s00253-012-4098-9en_US
dc.identifier.urihttp://hdl.handle.net/1808/23605
dc.description.abstractGenome sequencing of Aspergillus species including Aspergillus nidulans has revealed that there are far more secondary metabolite biosynthetic gene clusters than secondary metabolites isolated from these organisms. This implies that these organisms can produce additional secondary metabolites, which have not yet been elucidated. The A. nidulans genome contains 12 nonribosomal peptide synthetase (NRPS), one hybrid polyketide synthase/NRPS, and 14 NRPS-like genes. The only NRPS-like gene in A. nidulans with a known product is tdiA, which is involved in terrequinone A biosynthesis. To attempt to identify the products of these NRPS-like genes, we replaced the native promoters of the NRPS-like genes with the inducible alcohol dehydrogenase (alcA) promoter. Our results demonstrated that induction of the single NRPS-like gene AN3396.4 led to the enhanced production of microperfuranone. Furthermore, heterologous expression of AN3396.4 in Aspergillus niger confirmed that only one NRPS-like gene, AN3396.4, is necessary for the production of microperfuranone.en_US
dc.publisherSpringer Verlagen_US
dc.subjectAspergillus nidulansen_US
dc.subjectNonribosomal peptide synthetase-likeen_US
dc.subjectMicrooperfuranoneen_US
dc.subjectBiosynthesisen_US
dc.titleMolecular genetic analysis reveals that a nonribosomal peptide synthetase-like (NRPS-like) gene in Aspergillus nidulans is responsible for microperfuranone biosynthesisen_US
dc.typeArticleen_US
kusw.kuauthorEntwistle, Ruth
kusw.kuauthorAhuja, Manmeet
kusw.kuauthorOakley, Berl R.
kusw.kudepartmentMolecular Biosciencesen_US
dc.identifier.doi10.1007/s00253-012-4098-9en_US
dc.identifier.orcidhttps://orcid.org/0000-0003-4866-0326
kusw.oaversionScholarly/refereed, author accepted manuscripten_US
kusw.oapolicyThis item meets KU Open Access policy criteria.en_US
dc.rights.accessrightsopenAccess


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