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dc.contributor.authorTewary, Sunil Kumar
dc.contributor.authorLiang, Lingfei
dc.contributor.authorLin, Zihan
dc.contributor.authorLynn, Anna Y.
dc.contributor.authorCotmore, Susan F.
dc.contributor.authorTattersall, Peter
dc.contributor.authorZhao, Haiyan
dc.contributor.authorTang, Liang
dc.date.accessioned2016-02-12T21:47:40Z
dc.date.available2016-02-12T21:47:40Z
dc.date.issued2016-02-01
dc.identifier.citationTewary, Sunil K., Lingfei Liang, Zihan Lin, Annie Lynn, Susan F. Cotmore, Peter Tattersall, Haiyan Zhao, and Liang Tang. "Structures of Minute Virus of Mice Replication Initiator Protein N-terminal Domain: Insights into DNA Nicking and Origin Binding." Virology 476 (2015): 61-71. http://dx.doi.org/10.1016/j.virol.2014.11.022en_US
dc.identifier.urihttp://hdl.handle.net/1808/20073
dc.descriptionThis is the author's accepted manuscript. The original is available at http://www.sciencedirect.com/science/article/pii/S0042682214005236en_US
dc.description.abstractMembers of the Parvoviridae family all encode a non-structural protein 1 (NS1) that directs replication of single-stranded viral DNA, packages viral DNA into capsid, and serves as a potent transcriptional activator. Here we report the X-ray structure of the minute virus of mice (MVM) NS1 N-terminal domain at 1.45 Å resolution, showing that sites for dsDNA binding, ssDNA binding and cleavage, nuclear localization, and other functions are integrated on a canonical fold of the histidine-hydrophobic-histidine superfamily of nucleases, including elements specific for this Protoparvovirus but distinct from its Bocaparvovirus or Dependoparvovirus orthologs. High resolution structural analysis reveals a nickase active site with an architecture that allows highly versatile metal ligand binding. The structures support a unified mechanism of replication origin recognition for homotelomeric and heterotelomeric parvoviruses, mediated by a basic-residue-rich hairpin and an adjacent helix in the initiator proteins and by tandem tetranucleotide motifs in the replication origins.en_US
dc.publisherElsevieren_US
dc.subjectParvovirusen_US
dc.subjectDNA replicationen_US
dc.subjectNon-structural protein 1en_US
dc.subjectSite-specific DNA bindingen_US
dc.subjectNickaseen_US
dc.subjectNucleaseen_US
dc.titleStructures of minute virus of mice replication initiator protein N-terminal domain: insights into DNA nicking and origin bindingen_US
dc.typeArticle
kusw.kuauthorTang, Liang
kusw.kudepartmentMolecular Biosciencesen_US
dc.identifier.doi10.1016/j.virol.2014.11.022
dc.identifier.orcidhttps://orcid.org/0000-0002-2659-3284 https://orcid.org/0000-0001-5812-1866
kusw.oaversionScholarly/refereed, author accepted manuscript
kusw.oapolicyThis item meets KU Open Access policy criteria.
dc.rights.accessrightsopenAccess


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