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    Structures of minute virus of mice replication initiator protein N-terminal domain: insights into DNA nicking and origin binding

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    tang_structures.pdf (2.758Mb)
    Issue Date
    2016-02-01
    Author
    Tewary, Sunil Kumar
    Liang, Lingfei
    Lin, Zihan
    Lynn, Anna Y.
    Cotmore, Susan F.
    Tattersall, Peter
    Zhao, Haiyan
    Tang, Liang
    Publisher
    Elsevier
    Type
    Article
    Article Version
    Scholarly/refereed, author accepted manuscript
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    Abstract
    Members of the Parvoviridae family all encode a non-structural protein 1 (NS1) that directs replication of single-stranded viral DNA, packages viral DNA into capsid, and serves as a potent transcriptional activator. Here we report the X-ray structure of the minute virus of mice (MVM) NS1 N-terminal domain at 1.45 Å resolution, showing that sites for dsDNA binding, ssDNA binding and cleavage, nuclear localization, and other functions are integrated on a canonical fold of the histidine-hydrophobic-histidine superfamily of nucleases, including elements specific for this Protoparvovirus but distinct from its Bocaparvovirus or Dependoparvovirus orthologs. High resolution structural analysis reveals a nickase active site with an architecture that allows highly versatile metal ligand binding. The structures support a unified mechanism of replication origin recognition for homotelomeric and heterotelomeric parvoviruses, mediated by a basic-residue-rich hairpin and an adjacent helix in the initiator proteins and by tandem tetranucleotide motifs in the replication origins.
    Description
    This is the author's accepted manuscript. The original is available at http://www.sciencedirect.com/science/article/pii/S0042682214005236
    URI
    http://hdl.handle.net/1808/20073
    DOI
    https://doi.org/10.1016/j.virol.2014.11.022
    Collections
    • Molecular Biosciences Scholarly Works [537]
    Citation
    Tewary, Sunil K., Lingfei Liang, Zihan Lin, Annie Lynn, Susan F. Cotmore, Peter Tattersall, Haiyan Zhao, and Liang Tang. "Structures of Minute Virus of Mice Replication Initiator Protein N-terminal Domain: Insights into DNA Nicking and Origin Binding." Virology 476 (2015): 61-71. http://dx.doi.org/10.1016/j.virol.2014.11.022

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    KU Libraries
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    Lawrence, KS 66045
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    Contact KU ScholarWorks
    785-864-8983
    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    785-864-8983

    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    Image Credits
     

     

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