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Research Centers: Recent submissions
Now showing items 161-180 of 241
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Effects of Paraquat-induced Oxidative Stress on the Neuronal Plasma Membrane Ca2+-ATPase
(Elsevier, 2009-08-26)Oxidative stress leads to the disruption of calcium homeostasis in brain neurons; however, the direct effects of oxidants on proteins that regulate intracellular calcium [Ca2+]i are not known. The calmodulin (CaM) -stimulated ... -
Crystal structure of native Anopheles gambiae Serpin-2, a negative regulator of melanization in mosquitoes
(Wiley, 2011-06)Serpins are the dominant group of protease inhibitors in metazoans that control a wide variety of biological processes including major innate immune reactions. One of these inhibitors, SRPN2, controls melanization in ... -
Specific Inhibition of p97/VCP ATPase and Kinetic Analysis Demonstrate Interaction between D1 and D2 ATPase domains
(Elsevier, 2014-05-27)The p97 AAA (ATPase associated with diverse cellular activities), also called VCP (valosin-containing protein), is an important therapeutic target for cancer and neurodegenerative diseases. p97 forms a hexamer composed of ... -
Side-Chain Conformational Changes upon Protein-Protein Association
(Elsevier, 2011-02-25)Conformational changes upon protein-protein association are the key element of the binding mechanism. The study presents a systematic large-scale analysis of such conformational changes in the side chains. The results ... -
Fluorogenic tagging of protein 3-nitrotyrosine with 4-(aminomethyl)benzenesulfonate (ABS) in tissues: a useful alternative to immunohistochemistry for fluorescence microscopy imaging of protein nitration
(Elsevier, 2013-11-15)Protein tyrosine nitration is a common biomarker of biological aging and diverse pathologies associated with the excessive formation of reactive oxygen and nitrogen species. Recently, we suggested a novel fluorogenic ... -
HAb18G/CD147 Promotes pSTAT3-Mediated Pancreatic Cancer Development via CD44s
(American Association for Cancer Research, 2014-12-15)Purpose STAT3 plays a critical role in initiation and progression of pancreatic cancer. However, therapeutically targeting STAT3 is failure in clinic. We previously identified HAb18G/CD147 as an effective target for ... -
Titration-based screening for evaluation of natural product extracts: identification of an aspulvinone family of luciferase inhibitors
(Elsevier, 2012-11-23)The chemical diversity of nature has tremendous potential for discovery of new molecular probes and medicinal agents. However, sensitivity of HTS assays to interfering components of crude extracts derived from plants, ... -
Firefly luciferase in chemical biology: A compendium of inhibitors, mechanistic evaluation of chemotypes, and suggested use as a reporter
(Elsevier, 2012-08-24)Firefly luciferase (FLuc) is frequently used as a reporter in high-throughput screening assays owing to the exceptional sensitivity, dynamic range, and rapid measurement that bioluminescence affords. However, interaction ... -
MinD-dependent conformational changes in MinE required for the Min oscillator to spatially regulate cytokinesis
(Elsevier, 2011-08-05)MinD recruits MinE to the membrane leading to a coupled oscillation required for spatial regulation of the cytokinetic Z ring in E. coli. How these proteins interact, however, is not clear since the MinD binding regions ... -
NR2B-dependent cyclophilin D translocation suppresses the recovery of synaptic transmission after oxygen-glucose deprivation
(Elsevier, 2015-07-27)N-methyl d-aspartate receptor (NMDA) subunit 2B (NR2B)-containing NMDA receptors and mitochondrial protein cyclophilin D (CypD) are well characterized in mediating neuronal death after ischemia, respectively. However, ... -
Multi-faced neuroprotectice effects of Ginsenoside Rg1 in an Alzheimer mouse model
(Elsevier, 2011-10-12)There has been no extensive characterization of the effects of Ginsenoside Rg1, a pharmacological active component purified from the nature product ginseng, in an Alzheimer's disease mouse model. The well-characterized ... -
Inactivation of rabbit muscle glycogen phosphorylase b by peroxynitrite revisited: does the nitration of Tyr613 in the allosteric inhibition site control enzymatic function?
(Elsevier, 2008-12-27)There is increasing evidence that sequence-specific formation of 3-nitrotyrosine (3-NT) may cause functional changes in target proteins. Recently, the nitration of Tyr residues in glycogen phosphorylase b (Ph-b) was ... -
LC-MS/MS quantification of salvinorin A from biological fluids
(Royal Society of Chemistry, 2014-12-21)A facile method for quantifying the concentration of the powerful and widely available hallucinogen salvinorin A (a selective kappa opioid agonist) from non-human primate cerebrospinal fluid (CSF) and human plasma has been ... -
A Methodology for Simultaneous Fluorogenic Derivatization and Boronate Affinity Enrichment of 3-Nitrotyrosine Containing Peptides
(Elsevier Masson, 2012-11-15)We synthesized and characterized a new tagging reagent, (3R,4S)-1-(4-(aminomethyl)phenylsulfonyl)pyrrolidine-3,4-diol (APPD), for the selective fluorogenic derivatization of 3-nitrotyrosine (3-NT) residues in peptides ... -
A label-free mass spectrometry method for the quantification of protein isotypes
(Elsevier Masson, 2013-07-15)Successful quantitative mass spectrometry (MS) requires strategies to link the mass spectrometer response to the analyte abundance, with the response being dependent on more factors than just analyte abundance. Label-dependent ... -
Maturation of the proteasome core particle induces an affinity switch that controls regulatory particle association
(Nature Publishing Group, 2015-03-16)Proteasome assembly is a complex process, requiring 66 subunits distributed over several subcomplexes to associate in a coordinated fashion. Ten proteasome-specific chaperones have been identified that assist in this ... -
Opioid Receptor Probes Derived from Cycloaddition of the Hallucinogen Natural Product Salvinorin A
(Journal of Natural Products, 2011-02-21)As part of our continuing efforts toward more fully understanding the structure−activity relationships of the neoclerodane diterpene salvinorin A, we report the synthesis and biological characterization of unique cycloadducts ... -
Antiproliferative Withanolides from Datura wrightii
(Journal of Natural Products, 2012-12-19)A new withanolide, named withawrightolide (1), and four known withanolides (2−5) were isolated from the aerial parts of Datura wrightii. The structure of compound 1 was elucidated through 2D NMR and other spectroscopic ... -
Transmembrane Helix Assembly by Window Exchange Umbrella Sampling
(American Physical Society, 2012-03-08)A method of window exchange umbrella sampling molecular dynamics simulation is employed for transmembrane helix assembly. An analytical expression for the average acceptance probability between neighboring windows is derived ... -
Depressive Symptoms Affect Working Memory in Healthy Older Adult Hispanics
(OMICS International, 2015-09-27)Objectives: Low and middle income nations will experience an unprecedented growth of the elderly population and subsequent increase in age-related neurological disorders. Worldwide prevalence and incidence of all-types of ...