ATTENTION: The software behind KU ScholarWorks is being upgraded to a new version. Starting July 15th, users will not be able to log in to the system, add items, nor make any changes until the new version is in place at the end of July. Searching for articles and opening files will continue to work while the system is being updated.
If you have any questions, please contact Marianne Reed at mreed@ku.edu .
beta-tubulin C354 mutations that severely decrease microtubule dynamics do not prevent nuclear migration in yeast
dc.contributor.author | Bode, Claudia Janelle | |
dc.contributor.author | Himes, Richard H. | |
dc.contributor.author | Bloom, Kerry S. | |
dc.contributor.author | Suprenant, Kathy A. | |
dc.contributor.author | Pearson, Chad G. | |
dc.contributor.author | Thrower, Douglas A. | |
dc.contributor.author | Gupta, Mohan L., Jr. | |
dc.date.accessioned | 2008-01-02T19:26:07Z | |
dc.date.available | 2008-01-02T19:26:07Z | |
dc.date.issued | 2002-08 | |
dc.identifier.citation | Gupta, ML; Bode, CJ; Thrower, DA; Pearson, CG; Suprenant, KA; Bloom, KS; Himes, RH. beta-tubulin C354 mutations that severely decrease microtubule dynamics do not prevent nuclear migration in yeast. MOLECULAR BIOLOGY OF THE CELL. Aug 2002. 13(8): 2919-2932. | |
dc.identifier.uri | http://hdl.handle.net/1808/1791 | |
dc.description.abstract | Microtubule dynamics are influenced by interactions of microtubules with cellular factors and by changes in the primary sequence of the tubulin molecule. Mutations of yeast beta-tubulin C354, which is located near the binding site of some antimitotic compounds, reduce microtubule dynamicity greater than 90% in vivo and in vitro. The resulting intrinsically stable microtubules allowed us to determine which, if any, cellular processes are dependent on dynamic microtubules. The average number of cytoplasmic microtubules decreased from 3 in wild-type to 1 in mutant cells. The single microtubule effectively located the bud site before bud emergence. Although spindles were positioned near the bud neck at the onset of anaphase, the mutant cells were deficient in preanaphase spindle alignment along the mother-bud axis. Spindle microtubule dynamics and spindle elongation rates were also severely depressed in the mutants. The pattern and extent of cytoplasmic microtubule dynamics modulation through the cell cycle may reveal the minimum dynamic properties required to support growth. The ability to alter intrinsic microtubule dynamics and determine the in vivo phenotype of cells expressing the mutant tubulin provides a critical advance in assessing the dynamic requirements of an essential gene function. | |
dc.language.iso | en_US | |
dc.publisher | American Society for Cell Biology | |
dc.title | beta-tubulin C354 mutations that severely decrease microtubule dynamics do not prevent nuclear migration in yeast | |
dc.type | Article | |
kusw.kuauthor | Suprenant, Kathy A. | |
dc.identifier.doi | 10.1091/mbc.E02-01-0003 | |
dc.rights.accessrights | openAccess |
Files in this item
This item appears in the following Collection(s)
-
Center for Environmentally Beneficial Catalysis Scholarly Works [3]
This collection contains publications by faculty affiliated with the Center for Environmentally Beneficial Catalysis. -
Molecular Biosciences Scholarly Works [617]