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    beta-tubulin C354 mutations that severely decrease microtubule dynamics do not prevent nuclear migration in yeast

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    2919.37.pdf (634.1Kb)
    Issue Date
    2002-08
    Author
    Bode, Claudia Janelle
    Himes, Richard H.
    Bloom, Kerry S.
    Suprenant, Kathy A.
    Pearson, Chad G.
    Thrower, Douglas A.
    Gupta, Mohan L., Jr.
    Publisher
    American Society for Cell Biology
    Type
    Article
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    Abstract
    Microtubule dynamics are influenced by interactions of microtubules with cellular factors and by changes in the primary sequence of the tubulin molecule. Mutations of yeast beta-tubulin C354, which is located near the binding site of some antimitotic compounds, reduce microtubule dynamicity greater than 90% in vivo and in vitro. The resulting intrinsically stable microtubules allowed us to determine which, if any, cellular processes are dependent on dynamic microtubules. The average number of cytoplasmic microtubules decreased from 3 in wild-type to 1 in mutant cells. The single microtubule effectively located the bud site before bud emergence. Although spindles were positioned near the bud neck at the onset of anaphase, the mutant cells were deficient in preanaphase spindle alignment along the mother-bud axis. Spindle microtubule dynamics and spindle elongation rates were also severely depressed in the mutants. The pattern and extent of cytoplasmic microtubule dynamics modulation through the cell cycle may reveal the minimum dynamic properties required to support growth. The ability to alter intrinsic microtubule dynamics and determine the in vivo phenotype of cells expressing the mutant tubulin provides a critical advance in assessing the dynamic requirements of an essential gene function.
    URI
    http://hdl.handle.net/1808/1791
    DOI
    https://doi.org/10.1091/mbc.E02-01-0003
    Collections
    • Center for Environmentally Beneficial Catalysis Scholarly Works [3]
    • Molecular Biosciences Scholarly Works [472]
    Citation
    Gupta, ML; Bode, CJ; Thrower, DA; Pearson, CG; Suprenant, KA; Bloom, KS; Himes, RH. beta-tubulin C354 mutations that severely decrease microtubule dynamics do not prevent nuclear migration in yeast. MOLECULAR BIOLOGY OF THE CELL. Aug 2002. 13(8): 2919-2932.

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    Contact KU ScholarWorks
    785-864-8983
    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    785-864-8983

    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    Image Credits
     

     

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