Drosophila nod protein binds preferentially to the plus ends of microtubules and promotes microtubule polymerization in vitro

Abstract

Nod, a nonmotile kinesinlike protein, plays a critical role in segregating achiasmate chromosomes during female meiosis. In addition to localizing to oocyte chromosomes, we show that functional full-length Nod-GFP (Nod(FL)-GFP) localizes to the posterior pole of the oocyte at stages 9-10A, as does kinesin heavy chain WHO, a plus end-directed motor. This posterior localization is abolished in grk mutants that no longer maintain the microtubule (MT) gradient in the oocyte. To test the hypothesis that Nod binds to the plus ends of MTs, we expressed and purified both full-length Nod (Nod(FL)) and a truncated form of Nod containing only the motorlike domain (Nod(318)) from Escherichia coli and assessed their interactions with MTs in vitro. Both Nod(FL) and Nod(318) demonstrate preferential binding to the ends of the MTs, displaying a strong preference for binding to the plus ends. When Nod(318)-GFP:MT collision complexes were trapped by glutaraldehyde fixation, the preference for binding to plus ends versus minus ends was 17:1. Nod(FL) and Nod(318) also promote MT polymerization in vitro in a time-dependent manner. The observation that Nod is preferentially localized to the plus ends of MTs and stimulates NIT polymerization suggests a mechanism for its function.