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    Drosophila nod protein binds preferentially to the plus ends of microtubules and promotes microtubule polymerization in vitro

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    Issue Date
    2005-11
    Author
    Hawley, R. Scott
    Gilbert, Susan P.
    Matthies, Heinrich J. G.
    Gustafson, Susan M.
    Sproul, Lisa R.
    Cui, Wei
    Publisher
    American Society for Cell Biology
    Type
    Article
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    Abstract
    Nod, a nonmotile kinesinlike protein, plays a critical role in segregating achiasmate chromosomes during female meiosis. In addition to localizing to oocyte chromosomes, we show that functional full-length Nod-GFP (Nod(FL)-GFP) localizes to the posterior pole of the oocyte at stages 9-10A, as does kinesin heavy chain WHO, a plus end-directed motor. This posterior localization is abolished in grk mutants that no longer maintain the microtubule (MT) gradient in the oocyte. To test the hypothesis that Nod binds to the plus ends of MTs, we expressed and purified both full-length Nod (Nod(FL)) and a truncated form of Nod containing only the motorlike domain (Nod(318)) from Escherichia coli and assessed their interactions with MTs in vitro. Both Nod(FL) and Nod(318) demonstrate preferential binding to the ends of the MTs, displaying a strong preference for binding to the plus ends. When Nod(318)-GFP:MT collision complexes were trapped by glutaraldehyde fixation, the preference for binding to plus ends versus minus ends was 17:1. Nod(FL) and Nod(318) also promote MT polymerization in vitro in a time-dependent manner. The observation that Nod is preferentially localized to the plus ends of MTs and stimulates NIT polymerization suggests a mechanism for its function.
    URI
    http://hdl.handle.net/1808/1790
    DOI
    https://doi.org/10.1091/mbc.E05-06-0582
    Collections
    • Molecular Biosciences Scholarly Works [573]
    Citation
    Cui, W; Sproul, LR; Gustafson, SM; Matthies, HJG; Gilbert, SP; Hawley, RS. Drosophila nod protein binds preferentially to the plus ends of microtubules and promotes microtubule polymerization in vitro. MOLECULAR BIOLOGY OF THE CELL. Nov 2005. 16(11): 5400-5409.

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    Contact KU ScholarWorks
    785-864-8983
    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    785-864-8983

    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    Image Credits
     

     

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