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dc.contributor.authorQuan, Selwyn
dc.contributor.authorKwota, Zakari
dc.contributor.authorDuong, Trang
dc.contributor.authorBalazsi, Gabor
dc.contributor.authorCooper, Tim F.
dc.contributor.authorMonds, Russell D.
dc.contributor.authorRay, J. Christian J.
dc.date.accessioned2015-05-01T21:33:54Z
dc.date.available2015-05-01T21:33:54Z
dc.date.issued2012-01-12
dc.identifier.citationQuan, S., Ray, J., Kwota, Z., Duong, T., Balázsi, G., Cooper, T., . . . Guttman, D. (2012). Adaptive Evolution of the Lactose Utilization Network in Experimentally Evolved Populations of Escherichia coli. PLoS Genetics, 8(1), E1002444-E1002444. http://www.dx.doi.org/10.1371/journal.pgen.1002444en_US
dc.identifier.urihttp://hdl.handle.net/1808/17561
dc.descriptionThis is the publisher's version, also available electronically from "http://journals.plos.org".en_US
dc.description.abstractAdaptation to novel environments is often associated with changes in gene regulation. Nevertheless, few studies have been able both to identify the genetic basis of changes in regulation and to demonstrate why these changes are beneficial. To this end, we have focused on understanding both how and why the lactose utilization network has evolved in replicate populations of Escherichia coli. We found that lac operon regulation became strikingly variable, including changes in the mode of environmental response (bimodal, graded, and constitutive), sensitivity to inducer concentration, and maximum expression level. In addition, some classes of regulatory change were enriched in specific selective environments. Sequencing of evolved clones, combined with reconstruction of individual mutations in the ancestral background, identified mutations within the lac operon that recapitulate many of the evolved regulatory changes. These mutations conferred fitness benefits in environments containing lactose, indicating that the regulatory changes are adaptive. The same mutations conferred different fitness effects when present in an evolved clone, indicating that interactions between the lac operon and other evolved mutations also contribute to fitness. Similarly, changes in lac regulation not explained by lac operon mutations also point to important interactions with other evolved mutations. Together these results underline how dynamic regulatory interactions can be, in this case evolving through mutations both within and external to the canonical lactose utilization network.en_US
dc.publisherPublic Library of Scienceen_US
dc.titleAdaptive evolution of the lactose utilization network in experimentally evolved populations of Escherichia colien_US
dc.typeArticle
kusw.kuauthorRay, J. Christian J.
kusw.kudepartmentMolecular Biosciencesen_US
dc.identifier.doi10.1371/journal.pgen.1002444
kusw.oaversionScholarly/refereed, publisher version
kusw.oapolicyThis item meets KU Open Access policy criteria.
dc.rights.accessrightsopenAccess


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