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    Adaptive evolution of the lactose utilization network in experimentally evolved populations of Escherichia coli

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    RayC_PLoSG_8(1)e1002444.pdf (1.909Mb)
    Issue Date
    2012-01-12
    Author
    Quan, Selwyn
    Kwota, Zakari
    Duong, Trang
    Balazsi, Gabor
    Cooper, Tim F.
    Monds, Russell D.
    Ray, J. Christian J.
    Publisher
    Public Library of Science
    Type
    Article
    Article Version
    Scholarly/refereed, publisher version
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    Abstract
    Adaptation to novel environments is often associated with changes in gene regulation. Nevertheless, few studies have been able both to identify the genetic basis of changes in regulation and to demonstrate why these changes are beneficial. To this end, we have focused on understanding both how and why the lactose utilization network has evolved in replicate populations of Escherichia coli. We found that lac operon regulation became strikingly variable, including changes in the mode of environmental response (bimodal, graded, and constitutive), sensitivity to inducer concentration, and maximum expression level. In addition, some classes of regulatory change were enriched in specific selective environments. Sequencing of evolved clones, combined with reconstruction of individual mutations in the ancestral background, identified mutations within the lac operon that recapitulate many of the evolved regulatory changes. These mutations conferred fitness benefits in environments containing lactose, indicating that the regulatory changes are adaptive. The same mutations conferred different fitness effects when present in an evolved clone, indicating that interactions between the lac operon and other evolved mutations also contribute to fitness. Similarly, changes in lac regulation not explained by lac operon mutations also point to important interactions with other evolved mutations. Together these results underline how dynamic regulatory interactions can be, in this case evolving through mutations both within and external to the canonical lactose utilization network.
    Description
    This is the publisher's version, also available electronically from "http://journals.plos.org".
    URI
    http://hdl.handle.net/1808/17561
    DOI
    https://doi.org/10.1371/journal.pgen.1002444
    Collections
    • Molecular Biosciences Scholarly Works [590]
    Citation
    Quan, S., Ray, J., Kwota, Z., Duong, T., Balázsi, G., Cooper, T., . . . Guttman, D. (2012). Adaptive Evolution of the Lactose Utilization Network in Experimentally Evolved Populations of Escherichia coli. PLoS Genetics, 8(1), E1002444-E1002444. http://www.dx.doi.org/10.1371/journal.pgen.1002444

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    Lawrence, KS 66045
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    Contact KU ScholarWorks
    785-864-8983
    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    785-864-8983

    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    Image Credits
     

     

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