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    Precursors to 16S and 23S ribosomal RNA from a ribonuclease III− strain of Escherichia coli contain intact RNase III processing sites

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    Issue Date
    1980
    Author
    Gegenheimer, Peter Albert
    Apirion, David
    Publisher
    Oxford University Press
    Type
    Article
    Article Version
    Scholarly/refereed, publisher version
    Metadata
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    Abstract
    Escherichia coli cells lacking the ribosomal RNA processing enzyme RNase III do not excise the normal RNA precursors pl6a (17S) and p23a from nascent rRNA transcripts. These cells produce, instead, slightly larger pl6b and p23b precursors. Digestion of pl6b or p23b rRNA with RNases A plus Tl yields double-stranded fragments composed of sequences located at both the 5′ and the 3′ end regions of the molecules. The terminal duplex, or stem, of p 16b contains sequences surrounding the site of RNase III processing which in wild-type cells produces pl6a rRNA: the p23b stem likewise contains an intact RNase III cleavage site. The results confirm our earlier prediction for the structure of rRNA transcripts, and also yield a definite secondary structure for the pl6 stem, which was not uniquely determined by the corresponding DNA sequence. These experiments demonstrate the absence of significant RNase III processing activity in rnc-105 strains of E. coli, and implicate the participation of another endonuclease(s) 1n rRNA processing in mutant and wild-type cells.
    Description
    This is the published version. Copyright 1980 by the Oxford University Press. All rights reserved.
    URI
    http://hdl.handle.net/1808/17343
    DOI
    https://doi.org/10.1093/nar/8.8.1873
    Collections
    • Molecular Biosciences Scholarly Works [590]
    Citation
    Gegenheimer, Peter, and David Apirion. "Precursors to 16S and 23S Ribosomal RNA from a Ribonuclease III Strain of Escherichia Coli Contain Intact RNase III Processing Sites." Nucleic Acids Research 8.8 (1980): 1873-891. http://dx.doi.org/10.1093/nar/8.8.1873.

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    Contact KU ScholarWorks
    785-864-8983
    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    785-864-8983

    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    Image Credits
     

     

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