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dc.contributor.authorGegenheimer, Peter Albert
dc.contributor.authorApirion, David
dc.identifier.citationGegenheimer, Peter, and David Apirion. "Precursors to 16S and 23S Ribosomal RNA from a Ribonuclease III Strain of Escherichia Coli Contain Intact RNase III Processing Sites." Nucleic Acids Research 8.8 (1980): 1873-891.
dc.descriptionThis is the published version. Copyright 1980 by the Oxford University Press. All rights reserved.en_US
dc.description.abstractEscherichia coli cells lacking the ribosomal RNA processing enzyme RNase III do not excise the normal RNA precursors pl6a (17S) and p23a from nascent rRNA transcripts. These cells produce, instead, slightly larger pl6b and p23b precursors. Digestion of pl6b or p23b rRNA with RNases A plus Tl yields double-stranded fragments composed of sequences located at both the 5′ and the 3′ end regions of the molecules. The terminal duplex, or stem, of p 16b contains sequences surrounding the site of RNase III processing which in wild-type cells produces pl6a rRNA: the p23b stem likewise contains an intact RNase III cleavage site. The results confirm our earlier prediction for the structure of rRNA transcripts, and also yield a definite secondary structure for the pl6 stem, which was not uniquely determined by the corresponding DNA sequence. These experiments demonstrate the absence of significant RNase III processing activity in rnc-105 strains of E. coli, and implicate the participation of another endonuclease(s) 1n rRNA processing in mutant and wild-type cells.en_US
dc.publisherOxford University Pressen_US
dc.titlePrecursors to 16S and 23S ribosomal RNA from a ribonuclease III− strain of Escherichia coli contain intact RNase III processing sitesen_US
kusw.kuauthorGegenheimer, Peter Albert
kusw.kudepartmentMolecular Biosciencesen_US
kusw.oanotesPer SHERPA/RoMEO, April 8, 2015: Journal: Nucleic Acids Research (ISSN: 0305-1048, ESSN: 1362-4962) [started 1996] RoMEO: This is a RoMEO green journal Listed in: DOAJ as an open access journal Author's Pre-print: green tick author can archive pre-print (ie pre-refereeing) Author's Post-print: cross author cannot archive post-print (ie final draft post-refereeing) Publisher's Version/PDF: green tick author can archive publisher's version/PDF General Conditions:

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