Crystallization and preliminary X-ray diffraction studies of d(ACGTAGCTACGT)2:[actinomycin D, (echinomycin)2] and d(ACGTAGCTACGT)2: [actinomycin D, (triostin A)2] complexes

Abstract

A DNA-multiple drug complex, d(ACGTAGCTACGT)2:[actinomycin D, (echinomycin)2] has been crystallized. The crystals belong to the monoclinic space group C2, with unit-cell parameters a = 85.6, b = 72.8, c = 56.6 Å, [beta] = 101.5° at 93 K and Z = 8. The crystal diffracted to 3.0 Å resolution along the DNA fiber axis and to 3.5 Å resolution in other directions. The Patterson maps indicate that all complexes in the crystal are oriented along their helical axes in the [10\bar 1] direction.