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dc.contributor.authorSuprenant, Kathy A.
dc.contributor.authorDentler, William L., Jr
dc.date.accessioned2015-03-09T20:51:31Z
dc.date.available2015-03-09T20:51:31Z
dc.date.issued1982-04-01
dc.identifier.citationSuprenant, K. A.; Dentler, William L., Jr. (1982). "Association between endocrine pancreatic secretory granules and in-vitro-assembled microtubules is dependent upon microtubule-associated proteins." Journal of Cell Biology, 93(1):164-174. http://www.dx.doi.org/10.1083/jcb.93.1.164.en_US
dc.identifier.issn0021-9525
dc.identifier.urihttp://hdl.handle.net/1808/17010
dc.descriptionThis is the publisher's version, also available electronically from http://jcb.rupress.org/content/93/1/164.en_US
dc.description.abstractBy use of dark-field light microscopy, secretory granules isolated from the anglerfish endocrine pancreas were observed to attach to and release from microtubules assembled in vitro from brain homogenates. Secretory granules only bound to microtubules assembled in the presence of microtubule-associated proteins (MAPs) and not to microtubules assembled from purified tubulin. The addition of a MAP fraction to purified tubulin restored secretory granule binding. The secretory granules were released from MAP-containing microtubules by the addition of Mg-ATP but not by other nucleotides. The number of secretory granules bound to MAP-containing microtubules was increased in the presence of cyclic AMP. In addition to the associations of secretory granules with microtubules, MAP-containing microtubules also associated with each other. These laterally associated microtubules were dispersed by the addition of Mg-ATP. Electron micrographs confirmed that the associations between MAP-containing microtubules and secretory granules as well as the associations of microtubules with one another were mediated by the high molecular weight MAPs known to project from the surface of in-vitro-assembled microtubules.en_US
dc.publisherRockefeller University Pressen_US
dc.titleAssociation between endocrine pancreatic secretory granules and in-vitro-assembled microtubules is dependent upon microtubule-associated proteinsen_US
dc.typeArticle
kusw.kuauthorDentler, William L., Jr
kusw.kuauthorSuprenant, Kathy A.
kusw.kudepartmentMolecular Biosciencesen_US
dc.identifier.doi10.1083/jcb.93.1.164
kusw.oaversionScholarly/refereed, publisher version
kusw.oapolicyThis item does not meet KU Open Access policy criteria.
dc.rights.accessrightsopenAccess


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