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    Association between endocrine pancreatic secretory granules and in-vitro-assembled microtubules is dependent upon microtubule-associated proteins

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    Dentler_1982.pdf (1.353Mb)
    Issue Date
    1982-04-01
    Author
    Suprenant, Kathy A.
    Dentler, William L., Jr
    Publisher
    Rockefeller University Press
    Type
    Article
    Article Version
    Scholarly/refereed, publisher version
    Metadata
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    Abstract
    By use of dark-field light microscopy, secretory granules isolated from the anglerfish endocrine pancreas were observed to attach to and release from microtubules assembled in vitro from brain homogenates. Secretory granules only bound to microtubules assembled in the presence of microtubule-associated proteins (MAPs) and not to microtubules assembled from purified tubulin. The addition of a MAP fraction to purified tubulin restored secretory granule binding. The secretory granules were released from MAP-containing microtubules by the addition of Mg-ATP but not by other nucleotides. The number of secretory granules bound to MAP-containing microtubules was increased in the presence of cyclic AMP. In addition to the associations of secretory granules with microtubules, MAP-containing microtubules also associated with each other. These laterally associated microtubules were dispersed by the addition of Mg-ATP. Electron micrographs confirmed that the associations between MAP-containing microtubules and secretory granules as well as the associations of microtubules with one another were mediated by the high molecular weight MAPs known to project from the surface of in-vitro-assembled microtubules.
    Description
    This is the publisher's version, also available electronically from http://jcb.rupress.org/content/93/1/164.
    URI
    http://hdl.handle.net/1808/17010
    DOI
    https://doi.org/10.1083/jcb.93.1.164
    ISSN
    0021-9525
    Collections
    • Molecular Biosciences Scholarly Works [581]
    Citation
    Suprenant, K. A.; Dentler, William L., Jr. (1982). "Association between endocrine pancreatic secretory granules and in-vitro-assembled microtubules is dependent upon microtubule-associated proteins." Journal of Cell Biology, 93(1):164-174. http://www.dx.doi.org/10.1083/jcb.93.1.164.

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    Contact KU ScholarWorks
    785-864-8983
    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    785-864-8983

    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    Image Credits
     

     

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