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dc.contributor.advisorhttps://orcid.org/0000-0002-2149-8990
dc.contributor.authorSattilaro, Richard F.
dc.contributor.authorDentler, William L., Jr
dc.contributor.authorLeCluyse, Edward L.
dc.date.accessioned2015-03-09T20:40:13Z
dc.date.available2015-03-09T20:40:13Z
dc.date.issued1981-08-01
dc.identifier.citationSattilaro, R. F.; Dentler, William L., Jr; LeCluyse, E. L. (1981). "Microtubule-associated proteins (MAPs) and the organization of actin filaments in vitro." Journal of Cell Biology, 90(2):467-473. http://www.dx.doi.org/10.1083/jcb.90.2.467.en_US
dc.identifier.issn0021-9525
dc.identifier.urihttp://hdl.handle.net/1808/17008
dc.descriptionThis is the publisher's version, also available electronically from http://jcb.rupress.org/content/90/2/467.en_US
dc.description.abstractWhen purified muscle actin was mixed with microtubule-associated proteins (MAPs) prepared from brain microtubules assembled in vitro, actin filaments were organized into discrete bundles, 26 nm in diameter. MAP-2 was the principal protein necessary for the formation of the bundles. Analysis of MAP-actin bundle formation by sedimentation and electrophoresis revealed the bundles to be composed of approximately 20% MAP-2 and 80% actin by weight. Transverse striations were observed to occur at 28-nm intervals along negatively stained MAP-actin bundles, and short projections, approximately 12 nm long and spaced at 28-nm intervals, were resolved by high-resolution metal shadowing. The formation of MAP-actin bundles was inhibited by millimolar concentrations of ATP, AMP-PCP (beta, gamma-methylene-adenosine triphosphate), and pyrophosphate but not by AMP, ADP, or GTP. The addition of ATP to a solution containing MAP-actin bundles resulted in the dissociation of the bundles into individual actin filaments; discrete particles, presumably MAP-2, were periodically attached along the splayed filaments. These results demonstrate that MAPs can bind to actin filaments and can induce the reversible formation of actin filament bundles in vitro.en_US
dc.publisherRockefeller University Pressen_US
dc.titleMicrotubule-associated proteins (MAPs) and the organization of actin filaments in vitroen_US
dc.typeArticle
kusw.kuauthorDentler, William L., Jr
kusw.kudepartmentMolecular Biosciencesen_US
dc.identifier.doi10.1083/jcb.90.2.467
kusw.oaversionScholarly/refereed, publisher version
kusw.oapolicyThis item does not meet KU Open Access policy criteria.
dc.rights.accessrightsopenAccess


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