dc.contributor.author | Sattilaro, Richard F. | |
dc.contributor.author | Dentler, William L., Jr | |
dc.contributor.author | LeCluyse, Edward L. | |
dc.date.accessioned | 2015-03-09T20:40:13Z | |
dc.date.available | 2015-03-09T20:40:13Z | |
dc.date.issued | 1981-08-01 | |
dc.identifier.citation | Sattilaro, R. F.; Dentler, William L., Jr; LeCluyse, E. L. (1981). "Microtubule-associated proteins (MAPs) and the organization of actin filaments in vitro." Journal of Cell Biology, 90(2):467-473. http://www.dx.doi.org/10.1083/jcb.90.2.467. | en_US |
dc.identifier.issn | 0021-9525 | |
dc.identifier.uri | http://hdl.handle.net/1808/17008 | |
dc.description | This is the publisher's version, also available electronically from http://jcb.rupress.org/content/90/2/467. | en_US |
dc.description.abstract | When purified muscle actin was mixed with microtubule-associated proteins (MAPs) prepared from brain microtubules assembled in vitro, actin filaments were organized into discrete bundles, 26 nm in diameter. MAP-2 was the principal protein necessary for the formation of the bundles. Analysis of MAP-actin bundle formation by sedimentation and electrophoresis revealed the bundles to be composed of approximately 20% MAP-2 and 80% actin by weight. Transverse striations were observed to occur at 28-nm intervals along negatively stained MAP-actin bundles, and short projections, approximately 12 nm long and spaced at 28-nm intervals, were resolved by high-resolution metal shadowing. The formation of MAP-actin bundles was inhibited by millimolar concentrations of ATP, AMP-PCP (beta, gamma-methylene-adenosine triphosphate), and pyrophosphate but not by AMP, ADP, or GTP. The addition of ATP to a solution containing MAP-actin bundles resulted in the dissociation of the bundles into individual actin filaments; discrete particles, presumably MAP-2, were periodically attached along the splayed filaments. These results demonstrate that MAPs can bind to actin filaments and can induce the reversible formation of actin filament bundles in vitro. | en_US |
dc.publisher | Rockefeller University Press | en_US |
dc.title | Microtubule-associated proteins (MAPs) and the organization of actin filaments in vitro | en_US |
dc.type | Article | |
kusw.kuauthor | Dentler, William L., Jr | |
kusw.kudepartment | Molecular Biosciences | en_US |
dc.identifier.doi | 10.1083/jcb.90.2.467 | |
dc.identifier.orcid | https://orcid.org/0000-0002-2149-8990 | |
kusw.oaversion | Scholarly/refereed, publisher version | |
kusw.oapolicy | This item does not meet KU Open Access policy criteria. | |
dc.rights.accessrights | openAccess | |