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    Microtubule-associated proteins (MAPs) and the organization of actin filaments in vitro

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    Dentler_1981.pdf (954.4Kb)
    Issue Date
    1981-08-01
    Author
    Sattilaro, Richard F.
    Dentler, William L., Jr
    LeCluyse, Edward L.
    Publisher
    Rockefeller University Press
    Type
    Article
    Article Version
    Scholarly/refereed, publisher version
    Metadata
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    Abstract
    When purified muscle actin was mixed with microtubule-associated proteins (MAPs) prepared from brain microtubules assembled in vitro, actin filaments were organized into discrete bundles, 26 nm in diameter. MAP-2 was the principal protein necessary for the formation of the bundles. Analysis of MAP-actin bundle formation by sedimentation and electrophoresis revealed the bundles to be composed of approximately 20% MAP-2 and 80% actin by weight. Transverse striations were observed to occur at 28-nm intervals along negatively stained MAP-actin bundles, and short projections, approximately 12 nm long and spaced at 28-nm intervals, were resolved by high-resolution metal shadowing. The formation of MAP-actin bundles was inhibited by millimolar concentrations of ATP, AMP-PCP (beta, gamma-methylene-adenosine triphosphate), and pyrophosphate but not by AMP, ADP, or GTP. The addition of ATP to a solution containing MAP-actin bundles resulted in the dissociation of the bundles into individual actin filaments; discrete particles, presumably MAP-2, were periodically attached along the splayed filaments. These results demonstrate that MAPs can bind to actin filaments and can induce the reversible formation of actin filament bundles in vitro.
    Description
    This is the publisher's version, also available electronically from http://jcb.rupress.org/content/90/2/467.
    URI
    http://hdl.handle.net/1808/17008
    DOI
    https://doi.org/10.1083/jcb.90.2.467
    ISSN
    0021-9525
    Collections
    • Molecular Biosciences Scholarly Works [581]
    Citation
    Sattilaro, R. F.; Dentler, William L., Jr; LeCluyse, E. L. (1981). "Microtubule-associated proteins (MAPs) and the organization of actin filaments in vitro." Journal of Cell Biology, 90(2):467-473. http://www.dx.doi.org/10.1083/jcb.90.2.467.

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    Contact KU ScholarWorks
    785-864-8983
    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    785-864-8983

    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    Image Credits
     

     

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