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    MIP-1α Induces Differential MAP Kinase Activation and IκB Gene Expression in Human B Lymphocytes

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    Issue Date
    2004-07-24
    Author
    Teague, Ryan M.
    Harlan, Lisa M.
    Benedict, Stephen H.
    Chan, Marcia A.
    Publisher
    Mary Ann Liebert, Inc.
    Type
    Article
    Article Version
    Scholarly/refereed, publisher version
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    Abstract
    The chemokine macrophage inflammatory protein-1α (MIP-1α) stimulates migration of B cells and affects B cell immunoglobulin production. However, the molecular mechanisms by which MIP-1α modulates these biologic effects have not been completely defined. Previously, we demonstrated that treatment of B cells with MIP-1α induced the transcription factor, nuclear factor (NF)-κB, to bind to DNA, concomitant with the degradation of IκBα, a cytoplasmic inhibitor of NF-κB activation. Here, we report that MIP-1α treatment of tonsil B cells induced IκB gene expression that was dependent on MIP-1α-mediated activation of a pathway(s) involving NF-κB and phosphatidylinositol-3 kinase (PI3K). The NF-κB pathway is understood to be controlled in an autoregulatory fashion, so expression of IκB is thought to provide a means by which B cells modulate this pathway after stimulation with MIP-1α. Although the idea of NF-κB autoregulation is not novel, this is the first report to suggest the regulation of B cell gene expression by MIP-1α. In addition, we observed the activation of Jun N-terminal kinase (JNK) and p38 mitogenic-activated protein kinase (MAPK), but not extracellular signal-related kinase (ERK) in response to MIP-1α. Although p38 and NF-κB activity were both necessary for B cell migration, IκB gene expression was not affected by p38 inhibition, suggesting that p38 is involved in a separate MIP-1α-mediated signal transduction pathway.
    URI
    http://hdl.handle.net/1808/15798
    DOI
    https://doi.org/10.1089/1079990041535656
    Collections
    • Molecular Biosciences Scholarly Works [590]
    Citation
    Ryan M. Teague, Lisa M. Harlan, Stephen H. Benedict, and Marcia A. Chan. Journal of Interferon & Cytokine Research. July 2004, 24(7): 403-410. http://dx.doi.org/10.1089/1079990041535656.

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    Lawrence, KS 66045
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    Contact KU ScholarWorks
    785-864-8983
    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    785-864-8983

    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    Image Credits
     

     

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