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dc.contributor.authorTeague, Ryan M.
dc.contributor.authorHarlan, Lisa M.
dc.contributor.authorBenedict, Stephen H.
dc.contributor.authorChan, Marcia A.
dc.date.accessioned2014-11-18T21:20:42Z
dc.date.available2014-11-18T21:20:42Z
dc.date.issued2004-07-24
dc.identifier.citationRyan M. Teague, Lisa M. Harlan, Stephen H. Benedict, and Marcia A. Chan. Journal of Interferon & Cytokine Research. July 2004, 24(7): 403-410. http://dx.doi.org/10.1089/1079990041535656.
dc.identifier.urihttp://hdl.handle.net/1808/15798
dc.description.abstractThe chemokine macrophage inflammatory protein-1α (MIP-1α) stimulates migration of B cells and affects B cell immunoglobulin production. However, the molecular mechanisms by which MIP-1α modulates these biologic effects have not been completely defined. Previously, we demonstrated that treatment of B cells with MIP-1α induced the transcription factor, nuclear factor (NF)-κB, to bind to DNA, concomitant with the degradation of IκBα, a cytoplasmic inhibitor of NF-κB activation. Here, we report that MIP-1α treatment of tonsil B cells induced IκB gene expression that was dependent on MIP-1α-mediated activation of a pathway(s) involving NF-κB and phosphatidylinositol-3 kinase (PI3K). The NF-κB pathway is understood to be controlled in an autoregulatory fashion, so expression of IκB is thought to provide a means by which B cells modulate this pathway after stimulation with MIP-1α. Although the idea of NF-κB autoregulation is not novel, this is the first report to suggest the regulation of B cell gene expression by MIP-1α. In addition, we observed the activation of Jun N-terminal kinase (JNK) and p38 mitogenic-activated protein kinase (MAPK), but not extracellular signal-related kinase (ERK) in response to MIP-1α. Although p38 and NF-κB activity were both necessary for B cell migration, IκB gene expression was not affected by p38 inhibition, suggesting that p38 is involved in a separate MIP-1α-mediated signal transduction pathway.
dc.publisherMary Ann Liebert, Inc.
dc.titleMIP-1α Induces Differential MAP Kinase Activation and IκB Gene Expression in Human B Lymphocytes
dc.typeArticle
kusw.kuauthorTeague, Ryan M.
kusw.kuauthorHarlan, Lisa M.
kusw.kuauthorBenedict, Stephen H.
kusw.kuauthorChan, Marcia A.
kusw.kudepartmentDepartment of Molecular Biosciences
kusw.oastatusna
dc.identifier.doi10.1089/1079990041535656
kusw.oaversionScholarly/refereed, publisher version
kusw.oapolicyThis item does not meet KU Open Access policy criteria.
dc.rights.accessrightsopenAccess


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