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dc.contributor.authorShaw, R. J.
dc.contributor.authorDoherty, D. E.
dc.contributor.authorRitter, A. G.
dc.contributor.authorBenedict, Stephen H.
dc.contributor.authorClark, R. A. F.
dc.date.accessioned2014-11-18T20:54:12Z
dc.date.available2014-11-18T20:54:12Z
dc.date.issued1990-11-01
dc.identifier.citationShaw. et, al. "Adherence-dependent increase in human monocyte PDGF(B) mRNA is associated with increases in c-fos, c-jun, and EGR2 mRNA." JCB vol. 111 no. 5 2139-2148 The Rockefeller University Press, http://dx.doi.org/10.1083/jcb.111.5.2139
dc.identifier.urihttp://hdl.handle.net/1808/15795
dc.description.abstractAdherence is an important initial step in the transition of a circulating monocyte to a tissue macrophage. This differentiation is accompanied by an augmented capacity to generate growth factors. We hypothesized that adherence itself might be an important trigger for a sequence of gene activation culminating in cells with increased mRNA encoding profibrotic growth factors such as platelet-derived growth factor B subunit (PDGF[B]) and transforming growth factor-beta (TGF-beta). After in vitro adherence, human monocytes had a biphasic increase in PDGF(B) mRNA with peaks at 6 h and 13 d. No increase in TGF-beta mRNA was observed. The 6-h increase in PDGF(B) mRNA was adherence dependent, and in addition, was abrogated when the cytoskeletal integrity was compromised by cytochalasin D. The 6-h increase in PDGF(B) mRNA was unaltered by adherence in the presence of the monocyte stimulus lipopolysaccharide. Adherence to either fibronectin or collagen-coated plastic had little consistent effect on PDGF(B) mRNA accumulation. The increased PDGF(B) mRNA observed in adherent monocytes was accompanied by increases in mRNAs of the early growth response genes c-fos (maximal at 20 min), c-jun, and EGR2 (maximal at 6-24 h). The increase in c-jun and EGR2, but not c-fos, mRNA was also abrogated by cytochalasin D. These observations suggest that adherence results in increases of c-fos, c-jun, EGR2, and PDGF(B) mRNA. In addition, the increases in c-jun, EGR2, and PDGF(B) may depend on cytoskeletal rearrangement. Modulation of these events at the time of adherence offers a mechanism by which differential priming of the cells may be accomplished.
dc.description.sponsorshipR. J. Shaw was supported by the Medical Research Council U.K. and a Prophit scholarship from the Royal College of Physicians U.K.D.E. Doherty was supported by a Veterans Administration Merit Review Award and National Institutes of Health grant (HL-01804). This work was supported by grants from the NIH to S. H. Benedict (GM 40767) and R. A. F. Clark (AM-31514, HL-27353); and from the American Cancer Society to S. H. Benedict (IM510).
dc.publisherThe Rockefeller University Press
dc.titleAdherence-dependent Increase in Human Monocyte PDGF(B) mRNA Is Associated with Increases in c-fos, c-jun, and EGR2 mRNA
dc.typeArticle
kusw.kuauthorBenedict, Stephen H.
kusw.kudepartmentDepartment of Molecular Biosciences
kusw.oastatusna
dc.identifier.doi10.1083/jcb.111.5.2139
kusw.oaversionScholarly/refereed, publisher version
kusw.oapolicyThis item does not meet KU Open Access policy criteria.
dc.rights.accessrightsopenAccess


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