dc.contributor.author | Shaw, R. J. | |
dc.contributor.author | Doherty, D. E. | |
dc.contributor.author | Ritter, A. G. | |
dc.contributor.author | Benedict, Stephen H. | |
dc.contributor.author | Clark, R. A. F. | |
dc.date.accessioned | 2014-11-18T20:54:12Z | |
dc.date.available | 2014-11-18T20:54:12Z | |
dc.date.issued | 1990-11-01 | |
dc.identifier.citation | Shaw. et, al. "Adherence-dependent increase in human monocyte PDGF(B) mRNA is associated with increases in c-fos, c-jun, and EGR2 mRNA." JCB vol. 111 no. 5 2139-2148 The Rockefeller University Press, http://dx.doi.org/10.1083/jcb.111.5.2139 | |
dc.identifier.uri | http://hdl.handle.net/1808/15795 | |
dc.description.abstract | Adherence is an important initial step in the transition of a circulating monocyte to a tissue macrophage. This differentiation is accompanied by an augmented capacity to generate growth factors. We hypothesized that adherence itself might be an important trigger for a sequence of gene activation culminating in cells with increased mRNA encoding profibrotic growth factors such as platelet-derived growth factor B subunit (PDGF[B]) and transforming growth factor-beta (TGF-beta). After in vitro adherence, human monocytes had a biphasic increase in PDGF(B) mRNA with peaks at 6 h and 13 d. No increase in TGF-beta mRNA was observed. The 6-h increase in PDGF(B) mRNA was adherence dependent, and in addition, was abrogated when the cytoskeletal integrity was compromised by cytochalasin D. The 6-h increase in PDGF(B) mRNA was unaltered by adherence in the presence of the monocyte stimulus lipopolysaccharide. Adherence to either fibronectin or collagen-coated plastic had little consistent effect on PDGF(B) mRNA accumulation. The increased PDGF(B) mRNA observed in adherent monocytes was accompanied by increases in mRNAs of the early growth response genes c-fos (maximal at 20 min), c-jun, and EGR2 (maximal at 6-24 h). The increase in c-jun and EGR2, but not c-fos, mRNA was also abrogated by cytochalasin D. These observations suggest that adherence results in increases of c-fos, c-jun, EGR2, and PDGF(B) mRNA. In addition, the increases in c-jun, EGR2, and PDGF(B) may depend on cytoskeletal rearrangement. Modulation of these events at the time of adherence offers a mechanism by which differential priming of the cells may be accomplished. | |
dc.description.sponsorship | R. J. Shaw was supported by the Medical Research Council U.K. and a
Prophit scholarship from the Royal College of Physicians U.K.D.E. Doherty
was supported by a Veterans Administration Merit Review Award
and National Institutes of Health grant (HL-01804). This work was supported
by grants from the NIH to S. H. Benedict (GM 40767) and R. A. F.
Clark (AM-31514, HL-27353); and from the American Cancer Society to
S. H. Benedict (IM510). | |
dc.publisher | The Rockefeller University Press | |
dc.title | Adherence-dependent Increase in Human Monocyte PDGF(B) mRNA Is Associated with Increases in c-fos, c-jun, and EGR2 mRNA | |
dc.type | Article | |
kusw.kuauthor | Benedict, Stephen H. | |
kusw.kudepartment | Department of Molecular Biosciences | |
kusw.oastatus | na | |
dc.identifier.doi | 10.1083/jcb.111.5.2139 | |
kusw.oaversion | Scholarly/refereed, publisher version | |
kusw.oapolicy | This item does not meet KU Open Access policy criteria. | |
dc.rights.accessrights | openAccess | |