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    Adherence-dependent Increase in Human Monocyte PDGF(B) mRNA Is Associated with Increases in c-fos, c-jun, and EGR2 mRNA

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    Issue Date
    1990-11-01
    Author
    Shaw, R. J.
    Doherty, D. E.
    Ritter, A. G.
    Benedict, Stephen H.
    Clark, R. A. F.
    Publisher
    The Rockefeller University Press
    Type
    Article
    Article Version
    Scholarly/refereed, publisher version
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    Abstract
    Adherence is an important initial step in the transition of a circulating monocyte to a tissue macrophage. This differentiation is accompanied by an augmented capacity to generate growth factors. We hypothesized that adherence itself might be an important trigger for a sequence of gene activation culminating in cells with increased mRNA encoding profibrotic growth factors such as platelet-derived growth factor B subunit (PDGF[B]) and transforming growth factor-beta (TGF-beta). After in vitro adherence, human monocytes had a biphasic increase in PDGF(B) mRNA with peaks at 6 h and 13 d. No increase in TGF-beta mRNA was observed. The 6-h increase in PDGF(B) mRNA was adherence dependent, and in addition, was abrogated when the cytoskeletal integrity was compromised by cytochalasin D. The 6-h increase in PDGF(B) mRNA was unaltered by adherence in the presence of the monocyte stimulus lipopolysaccharide. Adherence to either fibronectin or collagen-coated plastic had little consistent effect on PDGF(B) mRNA accumulation. The increased PDGF(B) mRNA observed in adherent monocytes was accompanied by increases in mRNAs of the early growth response genes c-fos (maximal at 20 min), c-jun, and EGR2 (maximal at 6-24 h). The increase in c-jun and EGR2, but not c-fos, mRNA was also abrogated by cytochalasin D. These observations suggest that adherence results in increases of c-fos, c-jun, EGR2, and PDGF(B) mRNA. In addition, the increases in c-jun, EGR2, and PDGF(B) may depend on cytoskeletal rearrangement. Modulation of these events at the time of adherence offers a mechanism by which differential priming of the cells may be accomplished.
    URI
    http://hdl.handle.net/1808/15795
    DOI
    https://doi.org/10.1083/jcb.111.5.2139
    Collections
    • Molecular Biosciences Scholarly Works [590]
    Citation
    Shaw. et, al. "Adherence-dependent increase in human monocyte PDGF(B) mRNA is associated with increases in c-fos, c-jun, and EGR2 mRNA." JCB vol. 111 no. 5 2139-2148 The Rockefeller University Press, http://dx.doi.org/10.1083/jcb.111.5.2139

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    Contact KU ScholarWorks
    785-864-8983
    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    785-864-8983

    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    Image Credits
     

     

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