dc.contributor.author | Gracheva, Elena O. | |
dc.contributor.author | Burdina, Anna O. | |
dc.contributor.author | Holgado, Andrea M. | |
dc.contributor.author | Berthelot-Grosjean, Martine | |
dc.contributor.author | Ackley, Brian D. | |
dc.contributor.author | Hadwiger, Gayla | |
dc.contributor.author | Nonet, Michael L. | |
dc.contributor.author | Weimer, Robby M. | |
dc.contributor.author | Richmond, Janet E. | |
dc.date.accessioned | 2014-10-06T18:40:50Z | |
dc.date.available | 2014-10-06T18:40:50Z | |
dc.date.issued | 2006-07-25 | |
dc.identifier.citation | Gracheva, Elena O. et al. (2006). "Tomosyn Inhibits Synaptic Vesicle Priming in Caenorhabditis elegans." PLOS Biology, 4(8):e261. http://www.dx.doi.org/10.1371/journal.pbio.0040261 | |
dc.identifier.issn | 1544-9173 | |
dc.identifier.uri | http://hdl.handle.net/1808/15184 | |
dc.description | This is the publisher's version, also available electronically from http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.0040261. | |
dc.description.abstract | Caenorhabditis elegans TOM-1 is orthologous to vertebrate tomosyn, a cytosolic syntaxin-binding protein implicated in the modulation of both constitutive and regulated exocytosis. To investigate how TOM-1 regulates exocytosis of synaptic vesicles in vivo, we analyzed C. elegans tom-1 mutants. Our electrophysiological analysis indicates that evoked postsynaptic responses at tom-1 mutant synapses are prolonged leading to a two-fold increase in total charge transfer. The enhanced response in tom-1 mutants is not associated with any detectable changes in postsynaptic response kinetics, neuronal outgrowth, or synaptogenesis. However, at the ultrastructural level, we observe a concomitant increase in the number of plasma membrane-contacting vesicles in tom-1 mutant synapses, a phenotype reversed by neuronal expression of TOM-1. Priming defective unc-13 mutants show a dramatic reduction in plasma membrane-contacting vesicles, suggesting these vesicles largely represent the primed vesicle pool at the C. elegans neuromuscular junction. Consistent with this conclusion, hyperosmotic responses in tom-1 mutants are enhanced, indicating the primed vesicle pool is enhanced. Furthermore, the synaptic defects of unc-13 mutants are partially suppressed in tom-1 unc-13 double mutants. These data indicate that in the intact nervous system, TOM-1 negatively regulates synaptic vesicle priming. | |
dc.publisher | Public Library of Science | |
dc.title | Tomosyn Inhibits Synaptic Vesicle Priming in Caenorhabditis elegans | |
dc.type | Article | |
kusw.kuauthor | Ackley, Brian D. | |
kusw.kudepartment | Molecular Biosciences | |
kusw.oastatus | fullparticipation | |
dc.identifier.doi | 10.1371/journal.pbio.0040261 | |
kusw.oaversion | Scholarly/refereed, publisher version | |
kusw.oapolicy | This item meets KU Open Access policy criteria. | |
dc.rights.accessrights | openAccess | |