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    Tomosyn Inhibits Synaptic Vesicle Priming in Caenorhabditis elegans

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    Ackley_2006.pdf (583.5Kb)
    Issue Date
    2006-07-25
    Author
    Gracheva, Elena O.
    Burdina, Anna O.
    Holgado, Andrea M.
    Berthelot-Grosjean, Martine
    Ackley, Brian D.
    Hadwiger, Gayla
    Nonet, Michael L.
    Weimer, Robby M.
    Richmond, Janet E.
    Publisher
    Public Library of Science
    Type
    Article
    Article Version
    Scholarly/refereed, publisher version
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    Abstract
    Caenorhabditis elegans TOM-1 is orthologous to vertebrate tomosyn, a cytosolic syntaxin-binding protein implicated in the modulation of both constitutive and regulated exocytosis. To investigate how TOM-1 regulates exocytosis of synaptic vesicles in vivo, we analyzed C. elegans tom-1 mutants. Our electrophysiological analysis indicates that evoked postsynaptic responses at tom-1 mutant synapses are prolonged leading to a two-fold increase in total charge transfer. The enhanced response in tom-1 mutants is not associated with any detectable changes in postsynaptic response kinetics, neuronal outgrowth, or synaptogenesis. However, at the ultrastructural level, we observe a concomitant increase in the number of plasma membrane-contacting vesicles in tom-1 mutant synapses, a phenotype reversed by neuronal expression of TOM-1. Priming defective unc-13 mutants show a dramatic reduction in plasma membrane-contacting vesicles, suggesting these vesicles largely represent the primed vesicle pool at the C. elegans neuromuscular junction. Consistent with this conclusion, hyperosmotic responses in tom-1 mutants are enhanced, indicating the primed vesicle pool is enhanced. Furthermore, the synaptic defects of unc-13 mutants are partially suppressed in tom-1 unc-13 double mutants. These data indicate that in the intact nervous system, TOM-1 negatively regulates synaptic vesicle priming.
    Description
    This is the publisher's version, also available electronically from http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.0040261.
    URI
    http://hdl.handle.net/1808/15184
    DOI
    https://doi.org/10.1371/journal.pbio.0040261
    ISSN
    1544-9173
    Collections
    • Molecular Biosciences Scholarly Works [590]
    Citation
    Gracheva, Elena O. et al. (2006). "Tomosyn Inhibits Synaptic Vesicle Priming in Caenorhabditis elegans." PLOS Biology, 4(8):e261. http://www.dx.doi.org/10.1371/journal.pbio.0040261

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    Contact KU ScholarWorks
    785-864-8983
    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    785-864-8983

    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    Image Credits
     

     

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