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    Oligomerization-dependent Regulation of Motility and Morphogenesis by the Collagen XVIII NC1/Endostatin Domain

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    Ackley_2001_Oligomerization.pdf (999.5Kb)
    Issue Date
    2001-03-19
    Author
    Kuo, Calvin J.
    LaMontagne, Kenneth R.
    Garcia-Cardeña, Guillermo
    Ackley, Brian D.
    Kalman, Daniel
    Park, Susan
    Christofferson, Rolf
    Kamihara, Junne
    Ding, Yuan-Hua
    Lo, Kin-Ming
    Gillies, Stephen
    Folkman, Judah
    Mulligan, Richard C.
    Javaherian, Kashi
    Publisher
    The Rockefeller University Press
    Type
    Article
    Article Version
    Scholarly/refereed, publisher version
    Metadata
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    Abstract
    Collagen XVIII (c18) is a triple helical endothelial/epithelial basement membrane protein whose noncollagenous (NC)1 region trimerizes a COOH-terminal endostatin (ES) domain conserved in vertebrates, Caenorhabditis elegans and Drosophila. Here, the c18 NC1 domain functioned as a motility-inducing factor regulating the extracellular matrix (ECM)-dependent morphogenesis of endothelial and other cell types. This motogenic activity required ES domain oligomerization, was dependent on rac, cdc42, and mitogen-activated protein kinase, and exhibited functional distinction from the archetypal motogenic scatter factors hepatocyte growth factor and macrophage stimulatory protein. The motility-inducing and mitogen-activated protein kinase–stimulating activities of c18 NC1 were blocked by its physiologic cleavage product ES monomer, consistent with a proteolysis-dependent negative feedback mechanism. These data indicate that the collagen XVIII NC1 region encodes a motogen strictly requiring ES domain oligomerization and suggest a previously unsuspected mechanism for ECM regulation of motility and morphogenesis.
    Description
    This is the publisher's version, also available electronically from http://jcb.rupress.org/content/152/6/1233.
    URI
    http://hdl.handle.net/1808/15178
    DOI
    https://doi.org/10.1083/jcb.152.6.1233
    ISSN
    0021-9525
    Collections
    • Molecular Biosciences Scholarly Works [606]
    Citation
    Kuo, Calvin J. 2001. "Oligomerization-dependent Regulation of Motility and Morphogenesis by the Collagen XVIII NC1/Endostatin Domain." Journal of Cell Biology, v. 1152, pp. 1233. http://www.dx.doi.org/10.1083/jcb.152.6.1233

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    Contact KU ScholarWorks
    785-864-8983
    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    785-864-8983

    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    Image Credits
     

     

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