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dc.contributor.authorParker, Taybor W.
dc.contributor.authorNeufeld, Kristi L.
dc.date.accessioned2022-09-13T19:03:40Z
dc.date.available2022-09-13T19:03:40Z
dc.date.issued2020-02-19
dc.identifier.citationParker, T.W., Neufeld, K.L. APC controls Wnt-induced β-catenin destruction complex recruitment in human colonocytes. Sci Rep 10, 2957 (2020). https://doi.org/10.1038/s41598-020-59899-zen_US
dc.identifier.urihttp://hdl.handle.net/1808/33467
dc.description.abstractWnt/β-catenin signaling is essential for intestinal homeostasis and is aberrantly activated in most colorectal cancers (CRC) through mutation of the tumor suppressor Adenomatous Polyposis Coli (APC). APC is an essential component of a cytoplasmic protein complex that targets β-catenin for destruction. Following Wnt ligand presentation, this complex is inhibited. However, a role for APC in this inhibition has not been shown. Here, we utilized Wnt3a-beads to locally activate Wnt co-receptors. In response, the endogenous β-catenin destruction complex reoriented toward the local Wnt cue in CRC cells with full-length APC, but not if APC was truncated or depleted. Non-transformed human colon epithelial cells displayed similar Wnt-induced destruction complex localization which appeared to be dependent on APC and less so on Axin. Our results expand the current model of Wnt/β-catenin signaling such that in response to Wnt, the β-catenin destruction complex: (1) maintains composition and binding to β-catenin, (2) moves toward the plasma membrane, and (3) requires full-length APC for this relocalization.en_US
dc.publisherNature Researchen_US
dc.rights© The Author(s) 2020. This article is licensed under a Creative Commons Attribution 4.0 International License.en_US
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en_US
dc.subjectColon canceren_US
dc.subjectExtracellular signalling moleculesen_US
dc.titleAPC controls Wnt-induced β-catenin destruction complex recruitment in human colonocytesen_US
dc.typeArticleen_US
kusw.kuauthorParker, Taybor W.
kusw.kuauthorNeufeld, Kristi L.
kusw.kudepartmentMolecular Biosciencesen_US
dc.identifier.doidoi.org/10.1038/s41598-020-59899-zen_US
dc.identifier.orcidhttps://orcid.org/0000-0002-3829-7554en_US
dc.identifier.orcidhttps://orcid.org/0000-0003-3653-9385en_US
kusw.oaversionScholarly/refereed, publisher versionen_US
kusw.oapolicyThis item meets KU Open Access policy criteria.en_US
dc.rights.accessrightsopenAccessen_US


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© The Author(s) 2020. This article is licensed under a Creative Commons Attribution 4.0 International License.
Except where otherwise noted, this item's license is described as: © The Author(s) 2020. This article is licensed under a Creative Commons Attribution 4.0 International License.