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dc.contributor.authorPerlmutter, Jessamyn I.
dc.contributor.authorMeyers, Jane E.
dc.contributor.authorBordenstein, Seth R.
dc.date.accessioned2022-01-31T17:18:19Z
dc.date.available2022-01-31T17:18:19Z
dc.date.issued2021-10-22
dc.identifier.citationPerlmutter, J. I., Meyers, J. E., & Bordenstein, S. R. (2021). A single synonymous nucleotide change impacts the male-killing phenotype of prophage WO gene wmk. eLife, 10, e67686. https://doi.org/10.7554/eLife.67686en_US
dc.identifier.urihttp://hdl.handle.net/1808/32477
dc.description.abstractWolbachia are the most widespread bacterial endosymbionts in animals. Within arthropods, these maternally transmitted bacteria can selfishly hijack host reproductive processes to increase the relative fitness of their transmitting females. One such form of reproductive parasitism called male killing, or the selective killing of infected males, is recapitulated to degrees by transgenic expression of the prophage WO-mediated killing (wmk) gene. Here, we characterize the genotype-phenotype landscape of wmk-induced male killing in D. melanogaster using transgenic expression. While phylogenetically distant wmk homologs induce no sex-ratio bias, closely-related homologs exhibit complex phenotypes spanning no death, male death, or death of all hosts. We demonstrate that alternative start codons, synonymous codons, and notably a single synonymous nucleotide in wmk can ablate killing. These findings reveal previously unrecognized features of transgenic wmk-induced killing and establish new hypotheses for the impacts of post-transcriptional processes in male killing variation. We conclude that synonymous sequence changes are not necessarily silent in nested endosymbiotic interactions with life-or-death consequences.en_US
dc.publishereLife Sciences Publicationsen_US
dc.rightsCopyright Perlmutter et al. This article is distributed under the terms of the Creative Commons Attribution License.en_US
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en_US
dc.subjectD. melanogasteren_US
dc.titleA single synonymous nucleotide change impacts the male-killing phenotype of prophage WO gene wmken_US
dc.typeArticleen_US
kusw.kuauthorPerlmutter, Jessamyn I.
kusw.kudepartmentMolecular Biosciencesen_US
kusw.oanotesPer Sherpa Romeo 01/31/2021:

eLife [Open panel below]Publication Information TitleeLife [English] ISSNsElectronic: 2050-084X URLhttp://www.elifesciences.org/ PublisherseLife Sciences Publications [Commercial Publisher] DOAJ Listinghttps://doaj.org/toc/2050-084X Requires APCYes [Data provided by DOAJ] [Open panel below]Publisher Policy Open Access pathways permitted by this journal's policy are listed below by article version. Click on a pathway for a more detailed view.

Published Version NoneCC BY Any Website, Journal Website OA PublishingThis pathway includes Open Access publishing EmbargoNo Embargo LicenceCC BY Location Any Website Journal Website ConditionsPublished source must be acknowledged
en_US
dc.identifier.doi10.7554/eLife.67686en_US
kusw.oaversionScholarly/refereed, publisher versionen_US
kusw.oapolicyThis item meets KU Open Access policy criteria.en_US
dc.identifier.pmidPMC8555981en_US
dc.rights.accessrightsopenAccessen_US


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Copyright Perlmutter et al. This article is distributed under the terms of the Creative Commons Attribution License.
Except where otherwise noted, this item's license is described as: Copyright Perlmutter et al. This article is distributed under the terms of the Creative Commons Attribution License.