Show simple item record

dc.contributor.authorWeidling, Ian W.
dc.contributor.authorWilkins, Heather M.
dc.contributor.authorKoppel, Scott J.
dc.contributor.authorHutfles, Lewis
dc.contributor.authorWang, Xiaowan
dc.contributor.authorKalani, Anuradha
dc.contributor.authorMenta, Blaise W.
dc.contributor.authorRyan, Benjamin
dc.contributor.authorPerez-Ortiz, Judit
dc.contributor.authorGamblin, Chris
dc.contributor.authorSwerdlow, Russell H.
dc.identifier.citationWeidling, I. W., Wilkins, H. M., Koppel, S. J., Hutfles, L., Wang, X., Kalani, A., Menta, B. W., Ryan, B., Perez-Ortiz, J., Gamblin, T. C., & Swerdlow, R. H. (2020). Mitochondrial DNA Manipulations Affect Tau Oligomerization. Journal of Alzheimer's disease : JAD, 77(1), 149–163.
dc.description.abstractBackground:Mitochondrial dysfunction and tau aggregation occur in Alzheimer’s disease (AD), and exposing cells or rodents to mitochondrial toxins alters their tau. Objective:To further explore how mitochondria influence tau, we measured tau oligomer levels in human neuronal SH-SY5Y cells with different mitochondrial DNA (mtDNA) manipulations. Methods:Specifically, we analyzed cells undergoing ethidium bromide-induced acute mtDNA depletion, ρ0 cells with chronic mtDNA depletion, and cytoplasmic hybrid (cybrid) cell lines containing mtDNA from AD subjects. Results:We found cytochrome oxidase activity was particularly sensitive to acute mtDNA depletion, evidence of metabolic re-programming in the ρ0 cells, and a relatively reduced mtDNA content in cybrids generated through AD subject mitochondrial transfer. In each case tau oligomer levels increased, and acutely depleted and AD cybrid cells also showed a monomer to oligomer shift. Conclusion:We conclude a cell’s mtDNA affects tau oligomerization. Overlapping tau changes across three mtDNA-manipulated models establishes the reproducibility of the phenomenon, and its presence in AD cybrids supports its AD-relevance.en_US
dc.publisherIOS Pressen_US
dc.rightsCopyright 2020 The Author(s).en_US
dc.subjectAlzheimer’s diseaseen_US
dc.subjectMitochondrial DNAen_US
dc.titleMitochondrial DNA Manipulations Affect Tau Oligomerizationen_US
kusw.kuauthorGamblin, T. Chris
kusw.kudepartmentMolecular Biosciencesen_US
kusw.oanotesPer Sherpa Romeo 01/20/2022:

Journal of Alzheimer's Disease [Open panel below]Publication Information TitleJournal of Alzheimer's Disease [English] ISSNsPrint: 1387-2877 URL PublishersIOS Press [Commercial Publisher] [Open panel below]Publisher Policy Open Access pathways permitted by this journal's policy are listed below by article version. Click on a pathway for a more detailed view.

Published Version [pathway a]

None Institutional Repository, Funder Designated Location, Institutional Website, +2 Published Version [pathway b]

NoneCC BYPMC PMC, Journal Website Published Version [pathway c]

NoneCC BY Institutional Repository, Subject Repository, PMC, +1 Accepted Version [pathway a]

None Institutional Repository, Subject Repository, Funder Designated Location, +2 EmbargoNo Embargo Copyright OwnerAuthors Location Author's Homepage Funder Designated Location Institutional Repository Institutional Website Subject Repository Conditions Published sources is acknowledged with citation Must link to published version with DOI Set statement to accompany
kusw.oaversionScholarly/refereed, author accepted manuscripten_US
kusw.oapolicyThis item meets KU Open Access policy criteria.en_US

Files in this item


This item appears in the following Collection(s)

Show simple item record