dc.contributor.author | Al-Hashimi, Hikmat | |
dc.contributor.author | Chiarelli, Travis | |
dc.contributor.author | Lundquist, Erik A. | |
dc.contributor.author | Buechner, Matthew | |
dc.date.accessioned | 2020-11-23T16:10:02Z | |
dc.date.available | 2020-11-23T16:10:02Z | |
dc.date.issued | 2019-03-18 | |
dc.identifier.citation | Al-Hashimi, H., Chiarelli, T., Lundquist, E. A., & Buechner, M. (2019). Novel exc Genes Involved in Formation of the Tubular Excretory Canals of Caenorhabditis elegans. G3 (Bethesda, Md.), 9(5), 1339–1353. https://doi.org/10.1534/g3.119.200626 | en_US |
dc.identifier.uri | http://hdl.handle.net/1808/30902 | |
dc.description | This work is licensed under a Creative Commons Attribution 4.0 International License. | en_US |
dc.description.abstract | Regulation of luminal diameter is critical to the function of small single-celled tubes, of which the seamless tubular excretory canals of Caenorhabditis elegans provide a tractable genetic model. Mutations in several sets of genes exhibit the Exc phenotype, in which canal luminal growth is visibly altered. Here, a focused reverse genomic screen of genes highly expressed in the canals found 18 genes that significantly affect luminal outgrowth or diameter. These genes encode novel proteins as well as highly conserved proteins involved in processes including gene expression, cytoskeletal regulation, and vesicular and transmembrane transport. In addition, two genes act as suppressors on a pathway of conserved genes whose products mediate vesicle movement from early to recycling endosomes. The results provide new tools for understanding the integration of cytoplasmic structure and physiology in forming and maintaining the narrow diameter of single-cell tubules. | en_US |
dc.description.sponsorship | KU Graduate Research Fund #2301847 | en_US |
dc.description.sponsorship | KU Graduate Research Fund #2144091 | en_US |
dc.description.sponsorship | NIH NS0090945 | en_US |
dc.description.sponsorship | NIH NS0095682 | en_US |
dc.description.sponsorship | NIH NS0076063 | en_US |
dc.description.sponsorship | NIH GM103638 | en_US |
dc.description.sponsorship | NIH Office of Research Infrastructure Programs (P40 OD010440) | en_US |
dc.publisher | Genetics Society of America | en_US |
dc.rights | Copyright © 2019 Al-Hashimi et al. | en_US |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | en_US |
dc.subject | Tubulogenesis | en_US |
dc.subject | Lumen formation | en_US |
dc.subject | Endosomes | en_US |
dc.subject | EXC-5 | en_US |
dc.subject | Charcot-Marie-Tooth Syndrome Type 4H | en_US |
dc.title | Novel exc Genes Involved in Formation of the Tubular Excretory Canals of Caenorhabditis elegans | en_US |
dc.type | Article | en_US |
kusw.kuauthor | Al-Hashimi, Hikmat | |
kusw.kuauthor | Chiarelli, Travis | |
kusw.kuauthor | Lundquist, Erik A. | |
kusw.kuauthor | Buechner, Matthew | |
kusw.kudepartment | Molecular Biosciences | en_US |
dc.identifier.doi | 10.1534/g3.119.200626 | en_US |
dc.identifier.orcid | https://orcid.org/0000-0001-5999-2874 | en_US |
dc.identifier.orcid | https://orcid.org/0000-0003-1378-9162 | en_US |
dc.identifier.orcid | https://orcid.org/0000-0001-6819-4815 | en_US |
dc.identifier.orcid | https://orcid.org/0000-0002-2103-9399 | en_US |
kusw.oaversion | Scholarly/refereed, publisher version | en_US |
kusw.oapolicy | This item meets KU Open Access policy criteria. | en_US |
dc.identifier.pmid | PMC6505153 | en_US |
dc.rights.accessrights | openAccess | en_US |