dc.contributor.author | Holmstrom, Erik D. | |
dc.contributor.author | Liu, Zhaowei | |
dc.contributor.author | Nettels, Daniel | |
dc.contributor.author | Best, Robert B. | |
dc.contributor.author | Schuler, Benjamin | |
dc.date.accessioned | 2020-11-19T16:08:56Z | |
dc.date.available | 2020-11-19T16:08:56Z | |
dc.date.issued | 2019-06-05 | |
dc.identifier.citation | Holmstrom, E. D., Liu, Z., Nettels, D., Best, R. B., & Schuler, B. (2019). Disordered RNA chaperones can enhance nucleic acid folding via local charge screening. Nature communications, 10(1), 2453. https://doi.org/10.1038/s41467-019-10356-0 | en_US |
dc.identifier.uri | http://hdl.handle.net/1808/30884 | |
dc.description | This work is licensed under a Creative Commons Attribution 4.0 International License. | en_US |
dc.description.abstract | RNA chaperones are proteins that aid in the folding of nucleic acids, but remarkably, many of these proteins are intrinsically disordered. How can these proteins function without a well-defined three-dimensional structure? Here, we address this question by studying the hepatitis C virus core protein, a chaperone that promotes viral genome dimerization. Using single-molecule fluorescence spectroscopy, we find that this positively charged disordered protein facilitates the formation of compact nucleic acid conformations by acting as a flexible macromolecular counterion that locally screens repulsive electrostatic interactions with an efficiency equivalent to molar salt concentrations. The resulting compaction can bias unfolded nucleic acids towards folding, resulting in faster folding kinetics. This potentially widespread mechanism is supported by molecular simulations that rationalize the experimental findings by describing the chaperone as an unstructured polyelectrolyte. | en_US |
dc.description.sponsorship | Swiss National Science Foundation | en_US |
dc.description.sponsorship | European Molecular Biology Organization | en_US |
dc.description.sponsorship | Intramural Research Program of the NIDDK at the National Institutes of Health | en_US |
dc.publisher | Nature Research | en_US |
dc.rights | © The Author(s) 2019 | en_US |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | en_US |
dc.subject | RNA | en_US |
dc.subject | Biophysics | en_US |
dc.subject | Computational biophysics | en_US |
dc.subject | Intrinsically disordered proteins | en_US |
dc.subject | Molecular biophysics | en_US |
dc.title | Disordered RNA chaperones can enhance nucleic acid folding via local charge screening | en_US |
dc.type | Article | en_US |
kusw.kuauthor | Holmstrom, Erik D. | |
kusw.kudepartment | Molecular Biosciences | en_US |
dc.identifier.doi | 10.1038/s41467-019-10356-0 | en_US |
dc.identifier.orcid | https://orcid.org/0000-0003-2624-0806 | en_US |
dc.identifier.orcid | https://orcid.org/0000-0001-8214-8882 | en_US |
dc.identifier.orcid | https://orcid.org/0000-0002-7893-3543 | en_US |
dc.identifier.orcid | https://orcid.org/0000-0002-5970-4251 | en_US |
kusw.oaversion | Scholarly/refereed, publisher version | en_US |
kusw.oapolicy | This item meets KU Open Access policy criteria. | en_US |
dc.identifier.pmid | PMC6549165 | en_US |
dc.rights.accessrights | openAccess | en_US |