dc.contributor.author | Ochs, Matthew E. | |
dc.contributor.author | Josephson, Matthew P. | |
dc.contributor.author | Lundquist, Erik A. | |
dc.date.accessioned | 2020-11-12T15:09:03Z | |
dc.date.available | 2020-11-12T15:09:03Z | |
dc.date.issued | 2020-05-12 | |
dc.identifier.citation | Ochs, M. E., Josephson, M. P., & Lundquist, E. A. (2020). The Predicted RNA-Binding Protein ETR-1/CELF1 Acts in Muscles To Regulate Neuroblast Migration in Caenorhabditis elegans. G3 (Bethesda, Md.), 10(7), 2365–2376. https://doi.org/10.1534/g3.120.401182 | en_US |
dc.identifier.uri | http://hdl.handle.net/1808/30839 | |
dc.description | This work is licensed under a Creative Commons Attribution 4.0 International License. | en_US |
dc.description.abstract | Neuroblast migration is a critical aspect of nervous system development (e.g., neural crest migration). In an unbiased forward genetic screen, we identified a novel player in neuroblast migration, the ETR-1/CELF1 RNA binding protein. CELF1 RNA binding proteins are involved in multiple aspects of RNA processing including alternative splicing, stability, and translation. We find that a specific mutation in alternatively-spliced exon 8 results in migration defects of the AQR and PQR neurons, and not the embryonic lethality and body wall muscle defects of complete knockdown of the locus. Surprisingly, ETR-1 was required in body wall muscle cells for AQR/PQR migration (i.e., it acts cell non-autonomously). Genetic interactions indicate that ETR-1 acts with Wnt signaling, either in the Wnt pathway or in a parallel pathway. Possibly, ETR-1 is involved in the production of a Wnt signal or a parallel signal by the body wall muscles that controls AQR and PQR neuronal migration. In humans, CELF1 is involved in a number of neuromuscular disorders. If the role of ETR-1/CELF1 is conserved, these disorders might also involve cell or neuronal migration. Finally, we describe a technique of amplicon sequencing to detect rare, cell-specific genome edits by CRISPR/Cas9 in vivo (CRISPR-seq) as an alternative to the T7E1 assay. | en_US |
dc.description.sponsorship | NIH P40 OD010440 | en_US |
dc.description.sponsorship | National Institute of General Medical Sciences (P20GM103638) | en_US |
dc.description.sponsorship | Madison and Lila Self Graduate Fellowship program | en_US |
dc.publisher | Genetics Society of America | en_US |
dc.rights | Copyright © 2020 Ochs et al. | en_US |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | en_US |
dc.subject | Etr-1 | en_US |
dc.subject | Celf1 | en_US |
dc.subject | C. elegans | en_US |
dc.subject | Q neuroblast | en_US |
dc.subject | Migration | en_US |
dc.subject | CRISPR-seq | en_US |
dc.title | The Predicted RNA-Binding Protein ETR-1/CELF1 Acts in Muscles To Regulate Neuroblast Migration in Caenorhabditis elegans | en_US |
dc.type | Article | en_US |
kusw.kuauthor | Ochs, Matthew E. | |
kusw.kuauthor | Josephson, Matthew P. | |
kusw.kuauthor | Lundquist, Erik A. | |
kusw.kudepartment | Molecular Biosciences | en_US |
dc.identifier.doi | 10.1534/g3.120.401182 | en_US |
dc.identifier.orcid | https://orcid.org/0000-0001-5405-8652 | en_US |
dc.identifier.orcid | https://orcid.org/0000-0002-7336-2622 | en_US |
dc.identifier.orcid | https://orcid.org/0000-0001-6819-4815 | en_US |
kusw.oaversion | Scholarly/refereed, publisher version | en_US |
kusw.oapolicy | This item meets KU Open Access policy criteria. | en_US |
dc.identifier.pmid | PMC7341121 | en_US |
dc.rights.accessrights | openAccess | en_US |