Show simple item record

dc.contributor.authorOchs, Matthew E.
dc.contributor.authorJosephson, Matthew P.
dc.contributor.authorLundquist, Erik A.
dc.date.accessioned2020-11-12T15:09:03Z
dc.date.available2020-11-12T15:09:03Z
dc.date.issued2020-05-12
dc.identifier.citationOchs, M. E., Josephson, M. P., & Lundquist, E. A. (2020). The Predicted RNA-Binding Protein ETR-1/CELF1 Acts in Muscles To Regulate Neuroblast Migration in Caenorhabditis elegans. G3 (Bethesda, Md.), 10(7), 2365–2376. https://doi.org/10.1534/g3.120.401182en_US
dc.identifier.urihttp://hdl.handle.net/1808/30839
dc.descriptionThis work is licensed under a Creative Commons Attribution 4.0 International License.en_US
dc.description.abstractNeuroblast migration is a critical aspect of nervous system development (e.g., neural crest migration). In an unbiased forward genetic screen, we identified a novel player in neuroblast migration, the ETR-1/CELF1 RNA binding protein. CELF1 RNA binding proteins are involved in multiple aspects of RNA processing including alternative splicing, stability, and translation. We find that a specific mutation in alternatively-spliced exon 8 results in migration defects of the AQR and PQR neurons, and not the embryonic lethality and body wall muscle defects of complete knockdown of the locus. Surprisingly, ETR-1 was required in body wall muscle cells for AQR/PQR migration (i.e., it acts cell non-autonomously). Genetic interactions indicate that ETR-1 acts with Wnt signaling, either in the Wnt pathway or in a parallel pathway. Possibly, ETR-1 is involved in the production of a Wnt signal or a parallel signal by the body wall muscles that controls AQR and PQR neuronal migration. In humans, CELF1 is involved in a number of neuromuscular disorders. If the role of ETR-1/CELF1 is conserved, these disorders might also involve cell or neuronal migration. Finally, we describe a technique of amplicon sequencing to detect rare, cell-specific genome edits by CRISPR/Cas9 in vivo (CRISPR-seq) as an alternative to the T7E1 assay.en_US
dc.description.sponsorshipNIH P40 OD010440en_US
dc.description.sponsorshipNational Institute of General Medical Sciences (P20GM103638)en_US
dc.description.sponsorshipMadison and Lila Self Graduate Fellowship programen_US
dc.publisherGenetics Society of Americaen_US
dc.rightsCopyright © 2020 Ochs et al.en_US
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en_US
dc.subjectEtr-1en_US
dc.subjectCelf1en_US
dc.subjectC. elegansen_US
dc.subjectQ neuroblasten_US
dc.subjectMigrationen_US
dc.subjectCRISPR-seqen_US
dc.titleThe Predicted RNA-Binding Protein ETR-1/CELF1 Acts in Muscles To Regulate Neuroblast Migration in Caenorhabditis elegansen_US
dc.typeArticleen_US
kusw.kuauthorOchs, Matthew E.
kusw.kuauthorJosephson, Matthew P.
kusw.kuauthorLundquist, Erik A.
kusw.kudepartmentMolecular Biosciencesen_US
dc.identifier.doi10.1534/g3.120.401182en_US
dc.identifier.orcidhttps://orcid.org/0000-0001-5405-8652en_US
dc.identifier.orcidhttps://orcid.org/0000-0002-7336-2622en_US
dc.identifier.orcidhttps://orcid.org/0000-0001-6819-4815en_US
kusw.oaversionScholarly/refereed, publisher versionen_US
kusw.oapolicyThis item meets KU Open Access policy criteria.en_US
dc.identifier.pmidPMC7341121en_US
dc.rights.accessrightsopenAccessen_US


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record

Copyright © 2020 Ochs et al.
Except where otherwise noted, this item's license is described as: Copyright © 2020 Ochs et al.