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dc.contributor.authorSoules, Katelyn R.
dc.contributor.authorLaBrie, Scott D.
dc.contributor.authorMay, Benjamin H.
dc.contributor.authorHefty, P. Scott
dc.identifier.citationSoules, K. R., LaBrie, S. D., May, B. H., & Hefty, P. S. (2020). Sigma 54-Regulated Transcription Is Associated with Membrane Reorganization and Type III Secretion Effectors during Conversion to Infectious Forms of Chlamydia trachomatis. mBio, 11(5), e01725-20.
dc.descriptionThis work is licensed under a Creative Commons Attribution 4.0 International License.en_US
dc.description.abstractChlamydia bacteria are obligate intracellular organisms with a phylum-defining biphasic developmental cycle that is intrinsically linked to its ability to cause disease. The progression of the chlamydial developmental cycle is regulated by the temporal expression of genes predominantly controlled by RNA polymerase sigma (σ) factors. Sigma 54 (σ54) is one of three sigma factors encoded by Chlamydia for which the role and regulon are unknown. CtcC is part of a two-component signal transduction system that is requisite for σ54 transcriptional activation. CtcC activation of σ54 requires phosphorylation, which relieves inhibition by the CtcC regulatory domain and enables ATP hydrolysis by the ATPase domain. Prior studies with CtcC homologs in other organisms have shown that expression of the ATPase domain alone can activate σ54 transcription. Biochemical analysis of CtcC ATPase domain supported the idea of ATP hydrolysis occurring in the absence of the regulatory domain, as well as the presence of an active-site residue essential for ATPase activity (E242). Using recently developed genetic approaches in Chlamydia to induce expression of the CtcC ATPase domain, a transcriptional profile was determined that is expected to reflect the σ54 regulon. Computational evaluation revealed that the majority of the differentially expressed genes were preceded by highly conserved σ54 promoter elements. Reporter gene analyses using these putative σ54 promoters reinforced the accuracy of the model of the proposed regulon. Investigation of the gene products included in this regulon supports the idea that σ54 controls expression of genes that are critical for conversion of Chlamydia from replicative reticulate bodies into infectious elementary bodies.en_US
dc.description.sponsorshipNIH T32 GM008545en_US
dc.description.sponsorshipNIH (AI126785)en_US
dc.publisherAmerican Society for Microbiologyen_US
dc.rightsCopyright © 2020 Soules et al.en_US
dc.subjectChlamydia trachomatisen_US
dc.subjectGene regulationen_US
dc.subjectSigma factorsen_US
dc.titleSigma 54-Regulated Transcription Is Associated with Membrane Reorganization and Type III Secretion Effectors during Conversion to Infectious Forms of Chlamydia trachomatisen_US
kusw.kuauthorSoules, Katelyn R.
kusw.kuauthorLaBrie, Scott D.
kusw.kuauthorMay, Benjamin H.
kusw.kuauthorHefty, P. Scott
kusw.kudepartmentMolecular Biosciencesen_US
kusw.oaversionScholarly/refereed, publisher versionen_US
kusw.oapolicyThis item meets KU Open Access policy criteria.en_US

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Copyright © 2020 Soules et al.
Except where otherwise noted, this item's license is described as: Copyright © 2020 Soules et al.