ATTENTION: The software behind KU ScholarWorks is being upgraded to a new version. Starting July 15th, users will not be able to log in to the system, add items, nor make any changes until the new version is in place at the end of July. Searching for articles and opening files will continue to work while the system is being updated.
If you have any questions, please contact Marianne Reed at mreed@ku.edu .
The Pheromone Module SteC-MkkB-MpkB-SteD-HamE Regulates Development, Stress Responses and Secondary Metabolism in Aspergillus fumigatus
dc.contributor.author | Frawley, Dean | |
dc.contributor.author | Stroe, Maria C. | |
dc.contributor.author | Oakley, Berl R. | |
dc.contributor.author | Heinekamp, Thorsten | |
dc.contributor.author | Straßburger, Maria | |
dc.contributor.author | Fleming, Alastair B. | |
dc.contributor.author | Brakhage, Axel A. | |
dc.contributor.author | Bayram, Özgür | |
dc.date.accessioned | 2020-06-18T20:27:42Z | |
dc.date.available | 2020-06-18T20:27:42Z | |
dc.date.issued | 2020-05-07 | |
dc.identifier.citation | Frawley, D., Stroe, M. C., Oakley, B. R., Heinekamp, T., Straßburger, M., Fleming, A. B., Brakhage, A. A., & Bayram, Ö. (2020). The Pheromone Module SteC-MkkB-MpkB-SteD-HamE Regulates Development, Stress Responses and Secondary Metabolism in Aspergillus fumigatus. Frontiers in microbiology, 11, 811. https://doi.org/10.3389/fmicb.2020.00811 | en_US |
dc.identifier.uri | http://hdl.handle.net/1808/30553 | |
dc.description | This work is licensed under a Creative Commons Attribution 4.0 International License. | en_US |
dc.description.abstract | In order for eukaryotes to efficiently detect and respond to environmental stimuli, a myriad of protein signaling pathways are utilized. An example of highly conserved signaling pathways in eukaryotes are the mitogen-activated protein kinase (MAPK) pathways. In fungi, MAPK pathways have been shown to regulate a diverse array of biological processes, such as asexual and sexual development, stress responses and the production of secondary metabolites (SMs). In the model fungus Aspergillus nidulans, a MAPK pathway known as the pheromone module is utilized to regulate both development and SM production. This signaling cascade consists of the three kinases SteC, MkkB, and MpkB, as well as the SteD adaptor protein and the HamE scaffold. In this study, homologs of each of these proteins have been identified in the opportunistic human pathogen A. fumigatus. By performing epitope tagging and mass spectrometry experiments, we have shown that these proteins form a pentameric complex, similar to what is observed in A. nidulans. This complex has been shown to assemble in the cytoplasm and MpkB enters the nucleus, where it would presumably interact with various transcription factors. Pheromone module mutant strains exhibit drastic reductions in asexual sporulation, vegetative growth rate and production of SMs, such as gliotoxin. Mutants also display increased sensitivity to cell wall and oxidative stress agents. Overall, these data provide evidence of the existence of a conserved MAP kinase signaling pathway in Aspergillus species and suggest that this pathway is critical for the regulation of fungal development and secondary metabolism. | en_US |
dc.publisher | Frontiers Media | en_US |
dc.rights | © 2020 Frawley, Stroe, Oakley, Heinekamp, Straßburger, Fleming, Brakhage and Bayram. | en_US |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | en_US |
dc.subject | Aspergillus fumigatus | en_US |
dc.subject | Gliotoxin | en_US |
dc.subject | Pheromone module | en_US |
dc.subject | Secondary metabolism | en_US |
dc.subject | MAP Kinases | en_US |
dc.subject | Stress responses | en_US |
dc.subject | Asexual sporulation | en_US |
dc.title | The Pheromone Module SteC-MkkB-MpkB-SteD-HamE Regulates Development, Stress Responses and Secondary Metabolism in Aspergillus fumigatus | en_US |
dc.type | Article | en_US |
kusw.kuauthor | Oakley, Berl R. | |
kusw.kudepartment | Molecular Biosciences | en_US |
dc.identifier.doi | 10.3389/fmicb.2020.00811 | en_US |
kusw.oaversion | Scholarly/refereed, publisher version | en_US |
kusw.oapolicy | This item meets KU Open Access policy criteria. | en_US |
dc.identifier.pmid | PMC7223695 | en_US |
dc.rights.accessrights | openAccess | en_US |