dc.contributor.author | Bastos, Rafael Wesley | |
dc.contributor.author | Valero, Clara | |
dc.contributor.author | Silva, Lilian Pereira | |
dc.contributor.author | Schoen, Taylor | |
dc.contributor.author | Drott, Milton | |
dc.contributor.author | Brauer, Verônica | |
dc.contributor.author | Silva-Rocha, Rafael | |
dc.contributor.author | Lind, Abigail | |
dc.contributor.author | Steenwyk, Jacob L. | |
dc.contributor.author | Rokas, Antonis | |
dc.contributor.author | Rodrigues, Fernando | |
dc.contributor.author | Resendiz-Sharpe, Agustin | |
dc.contributor.author | Lagrou, Katrien | |
dc.contributor.author | Marcet-Houben, Marina | |
dc.contributor.author | Gabaldón, Toni | |
dc.contributor.author | McDonnell, Erin | |
dc.contributor.author | Reid, Ian | |
dc.contributor.author | Tsang, Adrian | |
dc.contributor.author | Oakley, Berl R. | |
dc.contributor.author | Loures, Flávio Vieira | |
dc.contributor.author | Almeida, Fausto | |
dc.contributor.author | Huttenlocher, Anna | |
dc.contributor.author | Keller, Nancy P. | |
dc.contributor.author | Ries, Laure Nicolas Annick | |
dc.contributor.author | Goldman, Gustavo H. | |
dc.date.accessioned | 2020-06-18T18:30:08Z | |
dc.date.available | 2020-06-18T18:30:08Z | |
dc.date.issued | 2020-04-08 | |
dc.identifier.citation | Bastos, R. W., Valero, C., Silva, L. P., Schoen, T., Drott, M., Brauer, V., Silva-Rocha, R., Lind, A., Steenwyk, J. L., Rokas, A., Rodrigues, F., Resendiz-Sharpe, A., Lagrou, K., Marcet-Houben, M., Gabaldón, T., McDonnell, E., Reid, I., Tsang, A., Oakley, B. R., Loures, F. V., … Goldman, G. H. (2020). Functional Characterization of Clinical Isolates of the Opportunistic Fungal Pathogen Aspergillus nidulans. mSphere, 5(2), e00153-20. https://doi.org/10.1128/mSphere.00153-20 | en_US |
dc.identifier.uri | http://hdl.handle.net/1808/30543 | |
dc.description | This work is licensed under a Creative Commons Attribution 4.0 International License. | en_US |
dc.description.abstract | Aspergillus nidulans is an opportunistic fungal pathogen in patients with immunodeficiency, and virulence of A. nidulans isolates has mainly been studied in the context of chronic granulomatous disease (CGD), with characterization of clinical isolates obtained from non-CGD patients remaining elusive. This study therefore carried out a detailed biological characterization of two A. nidulans clinical isolates (CIs), obtained from a patient with breast carcinoma and pneumonia and from a patient with cystic fibrosis that underwent lung transplantation, and compared them to the reference, nonclinical FGSC A4 strain. Both CIs presented increased growth in comparison to that of the reference strain in the presence of physiologically relevant carbon sources. Metabolomic analyses showed that the three strains are metabolically very different from each other in these carbon sources. Furthermore, the CIs were highly susceptible to cell wall-perturbing agents but not to other physiologically relevant stresses. Genome analyses identified several frameshift variants in genes encoding cell wall integrity (CWI) signaling components. Significant differences in CWI signaling were confirmed by Western blotting among the three strains. In vivo virulence studies using several different models revealed that strain MO80069 had significantly higher virulence in hosts with impaired neutrophil function than the other strains. In summary, this study presents detailed biological characterization of two A. nidulans sensu stricto clinical isolates. Just as in Aspergillus fumigatus, strain heterogeneity exists in A. nidulans clinical strains that can define virulence traits. Further studies are required to fully characterize A. nidulans strain-specific virulence traits and pathogenicity. | en_US |
dc.publisher | American Society for Microbiology | en_US |
dc.rights | © 2020 Bastos et al. | en_US |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | en_US |
dc.subject | Aspergillus nidulans | en_US |
dc.subject | Clinical isolates | en_US |
dc.subject | Genome sequencing | en_US |
dc.subject | Metabolomics | en_US |
dc.title | Functional Characterization of Clinical Isolates of the Opportunistic Fungal Pathogen Aspergillus nidulans | en_US |
dc.type | Article | en_US |
kusw.kuauthor | Oakley, Berl R. | |
kusw.kudepartment | Molecular Biosciences | en_US |
dc.identifier.doi | 10.1128/mSphere.00153-20 | en_US |
dc.identifier.orcid | https://orcid.org/0000-0001-9715-2200 | en_US |
dc.identifier.orcid | https://orcid.org/0000-0001-6319-631X | en_US |
dc.identifier.orcid | https://orcid.org/0000-0002-8436-595X | en_US |
dc.identifier.orcid | https://orcid.org/0000-0002-7248-6551 | en_US |
dc.identifier.orcid | https://orcid.org/0000-0002-3782-3698 | en_US |
dc.identifier.orcid | https://orcid.org/0000-0002-4386-9473 | en_US |
dc.identifier.orcid | https://orcid.org/0000-0002-2986-350X | en_US |
kusw.oaversion | Scholarly/refereed, publisher version | en_US |
kusw.oapolicy | This item meets KU Open Access policy criteria. | en_US |
dc.identifier.pmid | PMC7142298 | en_US |
dc.rights.accessrights | OpenAccess | en_US |