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dc.contributor.authorLan, Lan
dc.contributor.authorXing, Minli
dc.contributor.authorKashipathy, Maithri
dc.contributor.authorDouglas, Justin
dc.contributor.authorGao, Philip
dc.contributor.authorBattaile, Kevin
dc.contributor.authorHanzlik, Robert P.
dc.contributor.authorLovell, Scott
dc.contributor.authorXu, Liang
dc.identifier.citationLan, L., Xing, M., Kashipathy, M., Douglas, J., Gao, P., Battaile, K., Hanzlik, R., Lovell, S., & Xu, L. (2020). Crystal and solution structures of human oncoprotein Musashi-2 N-terminal RNA recognition motif 1. Proteins, 88(4), 573–583.
dc.descriptionThis work is licensed under a Creative Commons Attribution 4.0 International License.en_US
dc.description.abstractMusashi‐2 (MSI2) belongs to Musashi family of RNA binding proteins (RBP). Like Musashi‐1 (MSI1), it is overexpressed in a variety of cancers and is a promising therapeutic target. Both MSI proteins contain two N‐terminal RNA recognition motifs and play roles in posttranscriptional regulation of target mRNAs. Previously, we have identified several inhibitors of MSI1, all of which bind to MSI2 as well. In order to design MSI2‐specific inhibitors and compare the differences of binding mode of the inhibitors, we set out to solve the structure of MSI2‐RRM1, the key motif that is responsible for the binding. Here, we report the crystal structure and the first NMR solution structure of MSI2‐RRM1, and compare these to the structures of MSI1‐RBD1 and other RBPs. A high degree of structural similarity was observed between the crystal and solution NMR structures. MSI2‐RRM1 shows a highly similar overall folding topology to MSI1‐RBD1 and other RBPs. The structural information of MSI2‐RRM1 will be helpful for understanding MSI2‐RNA interaction and for guiding rational drug design of MSI2‐specific inhibitors.en_US
dc.rights© 2019 The Authors. Proteins: Structure, Function, and Bioinformatics published by Wiley Periodicals, Inc.en_US
dc.subjectRNA‐binding proteinen_US
dc.subjectRNA‐binding pocketen_US
dc.subjectNuclear magnetic resonanceen_US
dc.titleCrystal and solution structures of human oncoprotein Musashi‐2 N‐terminal RNA recognition motif 1en_US
kusw.kuauthorLan, Lan
kusw.kuauthorXing, Minli
kusw.kuauthorKashipathy, Maithri
kusw.kuauthorDouglas, Justin
kusw.kuauthorGao, Philip
kusw.kuauthorHanzlik, Robert
kusw.kuauthorLovell, Scott
kusw.kuauthorXu, Liang
kusw.kudepartmentMolecular Biosciencesen_US
kusw.kudepartmentBio‐NMR Core Facilityen_US
kusw.kudepartmentProtein Structure Laboratoryen_US
kusw.kudepartmentMedicinal Chemistryen_US
kusw.kudepartmentRadiation Oncologyen_US
kusw.oaversionScholarly/refereed, author accepted manuscripten_US
kusw.oapolicyThis item meets KU Open Access policy criteria.en_US

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© 2019 The Authors. Proteins: Structure, Function, and Bioinformatics published by Wiley Periodicals, Inc.
Except where otherwise noted, this item's license is described as: © 2019 The Authors. Proteins: Structure, Function, and Bioinformatics published by Wiley Periodicals, Inc.