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dc.contributor.authorChapman, Joanne R.
dc.contributor.authorDowell, Maureen A.
dc.contributor.authorChan, Rosanna
dc.contributor.authorUnckless, Robert L.
dc.date.accessioned2020-06-16T20:47:47Z
dc.date.available2020-06-16T20:47:47Z
dc.date.issued2020-02-22
dc.identifier.citationChapman, J. R., Dowell, M. A., Chan, R., & Unckless, R. L. (2020). The Genetic Basis of Natural Variation in Drosophila melanogaster Immune Defense against Enterococcus faecalis. Genes, 11(2), 234. https://doi.org/10.3390/genes11020234en_US
dc.identifier.urihttp://hdl.handle.net/1808/30519
dc.descriptionThis work is licensed under a Creative Commons Attribution 4.0 International License.en_US
dc.description.abstractDissecting the genetic basis of natural variation in disease response in hosts provides insights into the coevolutionary dynamics of host-pathogen interactions. Here, a genome-wide association study of Drosophila melanogaster survival after infection with the Gram-positive entomopathogenic bacterium Enterococcus faecalis is reported. There was considerable variation in defense against E. faecalis infection among inbred lines of the Drosophila Genetics Reference Panel. We identified single nucleotide polymorphisms associated with six genes with a significant (p < 10−08, corresponding to a false discovery rate of 2.4%) association with survival, none of which were canonical immune genes. To validate the role of these genes in immune defense, their expression was knocked-down using RNAi and survival of infected hosts was followed, which confirmed a role for the genes krishah and S6k in immune defense. We further identified a putative role for the Bomanin gene BomBc1 (also known as IM23), in E. faecalis infection response. This study adds to the growing set of association studies for infection in Drosophila melanogaster and suggests that the genetic causes of variation in immune defense differ for different pathogens.en_US
dc.publisherMDPIen_US
dc.rights© 2020 by the authors.en_US
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en_US
dc.subjectGenome-wide association study (GWAS)en_US
dc.subjectInnate immunityen_US
dc.subjectImmune responseen_US
dc.subjectSystemic infectionen_US
dc.subjectToll pathwayen_US
dc.subjectGram-positive bacteriaen_US
dc.titleThe Genetic Basis of Natural Variation in Drosophila melanogaster Immune Defense against Enterococcus faecalisen_US
dc.typeArticleen_US
kusw.kuauthorChapman, Joanne R.
kusw.kuauthorDowell, Maureen A.
kusw.kuauthorChan, Rosanna
kusw.kuauthorUnckless, Robert L.
kusw.kudepartmentMolecular Biosciencesen_US
dc.identifier.doi10.3390/genes11020234en_US
dc.identifier.orcidhttps://orcid.org/0000-0001-6492-899Xen_US
dc.identifier.orcidhttps://orcid.org/0000-0001-8586-7137en_US
kusw.oaversionScholarly/refereed, publisher versionen_US
kusw.oapolicyThis item meets KU Open Access policy criteria.en_US
dc.identifier.pmidPMC7074548en_US
dc.rights.accessrightsopenAccessen_US


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Except where otherwise noted, this item's license is described as: © 2020 by the authors.