dc.contributor.author | Benomar, Saida | |
dc.contributor.author | Lansdon, Patrick | |
dc.contributor.author | Bender, Aaron M. | |
dc.contributor.author | Peterson, Blake R. | |
dc.contributor.author | Chandler, Josephine R. | |
dc.contributor.author | Ackley, Brian D. | |
dc.date.accessioned | 2020-06-15T20:11:30Z | |
dc.date.available | 2020-06-15T20:11:30Z | |
dc.date.issued | 2020-01-09 | |
dc.identifier.citation | Benomar, S., Lansdon, P., Bender, A. M., Peterson, B. R., Chandler, J. R., & Ackley, B. D. (2020). The C. elegans CHP1 homolog, pbo-1, functions in innate immunity by regulating the pH of the intestinal lumen. PLoS pathogens, 16(1), e1008134. https://doi.org/10.1371/journal.ppat.1008134 | en_US |
dc.identifier.uri | http://hdl.handle.net/1808/30501 | |
dc.description | This work is licensed under a Creative Commons Attribution 4.0 International License. | en_US |
dc.description.abstract | Caenorhabditis elegans are soil-dwelling nematodes and models for understanding innate immunity and infection. Previously, we developed a novel fluorescent dye (KR35) that accumulates in the intestine of C. elegans and reports a dynamic wave in intestinal pH associated with the defecation motor program. Here, we use KR35 to show that mutations in the Ca2+-binding protein, PBO-1, abrogate the pH wave, causing the anterior intestine to be constantly acidic. Surprisingly, pbo-1 mutants were also more susceptible to infection by several bacterial pathogens. We could suppress pathogen susceptibility in pbo-1 mutants by treating the animals with pH-buffering bicarbonate, suggesting the pathogen susceptibility is a function of the acidity of the intestinal pH. Furthermore, we use KR35 to show that upon infection by pathogens, the intestinal pH becomes neutral in a wild type, but less so in pbo-1 mutants. C. elegans is known to increase production of reactive oxygen species (ROS), such as H2O2, in response to pathogens, which is an important component of pathogen defense. We show that pbo-1 mutants exhibited decreased H2O2 in response to pathogens, which could also be partially restored in pbo-1 animals treated with bicarbonate. Ultimately, our results support a model whereby PBO-1 functions during infection to facilitate pH changes in the intestine that are protective to the host. | en_US |
dc.publisher | Public Library of Science | en_US |
dc.rights | © 2020 Benomar et al. | en_US |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | en_US |
dc.title | The C. elegans CHP1 homolog, pbo-1, functions in innate immunity by regulating the pH of the intestinal lumen | en_US |
dc.type | Article | en_US |
kusw.kuauthor | Benomar, Saida | |
kusw.kuauthor | Lansdon, Patrick | |
kusw.kuauthor | Bender, Aaron M. | |
kusw.kuauthor | Peterson, Blake R. | |
kusw.kuauthor | Chandler, Josephine R. | |
kusw.kudepartment | Molecular Biosciences | en_US |
kusw.kudepartment | Medicinal Chemistry | en_US |
dc.identifier.doi | 10.1371/journal.ppat.1008134 | en_US |
dc.identifier.orcid | https://orcid.org/0000-0003-2395-0343 | en_US |
dc.identifier.orcid | https://orcid.org/0000-0001-8251-3579 | en_US |
dc.identifier.orcid | https://orcid.org/0000-0001-8944-9813 | en_US |
dc.identifier.orcid | https://orcid.org/0000-0002-1257-2407 | en_US |
kusw.oaversion | Scholarly/refereed, publisher version | en_US |
kusw.oapolicy | This item meets KU Open Access policy criteria. | en_US |
dc.identifier.pmid | PMC6952083 | en_US |
dc.rights.accessrights | openAccess | en_US |