Abstract
The rising use of titanium dental implants has increased the prevalence of peri-implant disease that shortens their useful life. A growing view of peri-implant disease suggests that plaque accumulation and microbiome dysbiogenesis trigger a host immune inflammatory response that destroys soft and hard tissues supporting the implant. The incidence of peri-implant disease is difficult to estimate, but with over 3 million implants placed in the USA alone, and the market growing by 500,000 implants/year, such extensive use demands additional interceptive approaches. We report a water-based, nonsurgical approach to address peri-implant disease using a bifunctional peptide film, which can be applied during initial implant placement and later reapplied to existing implants to reduce bacterial growth. Bifunctional peptides are based upon a titanium binding peptide (TiBP) optimally linked by a spacer peptide to an antimicrobial peptide (AMP). We show herein that dental implant surfaces covered with a bifunctional peptide film kill bacteria. Further, using a simple protocol for cleaning implant surfaces fouled by bacteria, the surface can be effectively recoated with TiBP-AMP to regain an antimicrobial state. Fouling, cleansing, and rebinding was confirmed for up to four cycles with minimal loss of binding efficacy. After fouling, rebinding with a water-based peptide film extends control over the oral microbiome composition, providing a novel nonsurgical treatment for dental implants.
Description
This is a post-peer-review, pre-copyedit version of an article published in JOM Journal of the Minerals, Metals and Materials Society. The final authenticated version is available online at: http://dx.doi.org/10.1007/s11837-019-03334-w.
Citation
Wisdom, C., Chen, C., Yuca, E., Zhou, Y., Tamerler, C., & Snead, M. L. (2019). Repeatedly Applied Peptide Film Kills Bacteria on Dental Implants. JOM (Warrendale, Pa. : 1989), 71(4), 1271–1280. doi:10.1007/s11837-019-03334-w