Human oncoprotein Musashi-2 N-terminal RNA recognition motif backbone assignment and identification of RNA-binding pocket
dc.contributor.author | Lan, Lan | |
dc.contributor.author | Xing, Minli | |
dc.contributor.author | Douglas, Justin T. | |
dc.contributor.author | Gao, Philip | |
dc.contributor.author | Hanzlik, Robert P. | |
dc.contributor.author | Xu, Liang | |
dc.date.accessioned | 2018-11-16T22:17:05Z | |
dc.date.available | 2018-11-16T22:17:05Z | |
dc.date.issued | 2017-11-20 | |
dc.identifier.citation | Lan, L., Xing, M., Douglas, J. T., Gao, P., Hanzlik, R. P., & Xu, L. (2017). Human oncoprotein Musashi-2 N-terminal RNA recognition motif backbone assignment and identification of RNA-binding pocket. Oncotarget, 8(63), 106587. | en_US |
dc.identifier.uri | http://hdl.handle.net/1808/27397 | |
dc.description.abstract | RNA-binding protein Musashi-2 (MSI2) is a key regulator in stem cells, it is over-expressed in a variety of cancers and its higher expression is associated with poor prognosis. Like Musashi-1, it contains two N-terminal RRMs (RNA-recognition Motifs, also called RBDs (RNA-binding Domains)), RRM1 and RRM2, which mediate the binding to their target mRNAs. Previous studies have obtained the three-dimensional structures of the RBDs of Musashi-1 and the RBD1:RNA complex. Here we show the binding of MSI2-RRM1 to a 15nt Numb RNA in Fluorescence Polarization assay and time resolved Fluorescence Resonance Energy Transfer assay. Using nuclear magnetic resonance (NMR) spectroscopy we assigned the backbone resonances of MSI2-RRM1, and characterized the direct interaction of RRM1 to Numb RNA r(GUAGU). Our NMR titration and structure modeling studies showed that MSI2-RRM1 and MSI1-RBD1 have similar RNA binding events and binding pockets. This work adds significant information to MSI2-RRM1 structure and RNA binding pocket, and contributes to the development of MSI2 specific and MSI1/MSI2 dual inhibitors. | en_US |
dc.publisher | Impact Journals | en_US |
dc.rights | Lan et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | en_US |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/ | en_US |
dc.subject | RNA-binding protein | en_US |
dc.subject | RNA-binding pocket | en_US |
dc.subject | Nuclear magnetic resonance | en_US |
dc.subject | Backbone assignment | en_US |
dc.subject | Musashi | en_US |
dc.title | Human oncoprotein Musashi-2 N-terminal RNA recognition motif backbone assignment and identification of RNA-binding pocket | en_US |
dc.type | Article | en_US |
kusw.kuauthor | Lan, Lan | |
kusw.kuauthor | Xing, Minli | |
kusw.kuauthor | Douglas, Justin T. | |
kusw.kuauthor | Gao, Philip | |
kusw.kuauthor | Hanzlik, Robert P. | |
kusw.kuauthor | Xu, Liang | |
kusw.kudepartment | Molecular Biosciences | en_US |
dc.identifier.doi | 10.18632/oncotarget.22540 | en_US |
kusw.oaversion | Scholarly/refereed, publisher version | en_US |
kusw.oapolicy | This item meets KU Open Access policy criteria. | en_US |
dc.rights.accessrights | openAccess | en_US |
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Except where otherwise noted, this item's license is described as: Lan et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.