SUMOylation of DNA topoisomerase IIα regulates histone H3 kinase Haspin and H3 phosphorylation in mitosis
dc.contributor.author | Yoshida, Makoto Michael | |
dc.contributor.author | Ting, Lily | |
dc.contributor.author | Gygi, Steven P. | |
dc.contributor.author | Azuma, Yoshiaki | |
dc.date.accessioned | 2017-09-20T18:14:23Z | |
dc.date.available | 2017-09-20T18:14:23Z | |
dc.date.issued | 2016-06-20 | |
dc.identifier.citation | Yoshida, M. M., Ting, L., Gygi, S. P., & Azuma, Y. (2016). SUMOylation of DNA topoisomerase IIα regulates histone H3 kinase Haspin and H3 phosphorylation in mitosis. The Journal of Cell Biology, 213(6), 665–678. http://doi.org/10.1083/jcb.201511079 | en_US |
dc.identifier.uri | http://hdl.handle.net/1808/24972 | |
dc.description.abstract | DNA topoisomerase II (TOP2) plays a pivotal role in faithful chromosome separation through its strand-passaging activity that resolves tangled genomic DNA during mitosis. Additionally, TOP2 controls progression of mitosis by activating cell cycle checkpoints. Recent work showed that the enzymatically inert C-terminal domain (CTD) of TOP2 and its posttranslational modification are critical to this checkpoint regulation. However, the molecular mechanism has not yet been determined. By using Xenopus laevis egg extract, we found that SUMOylation of DNA topoisomerase IIα (TOP2A) CTD regulates the localization of the histone H3 kinase Haspin and phosphorylation of histone H3 at threonine 3 at the centromere, two steps known to be involved in the recruitment of the chromosomal passenger complex (CPC) to kinetochores in mitosis. Robust centromeric Haspin localization requires SUMOylated TOP2A CTD binding activity through SUMO-interaction motifs and the phosphorylation of Haspin. We propose a novel mechanism through which the TOP2 CTD regulates the CPC via direct interaction with Haspin at mitotic centromeres. | en_US |
dc.publisher | Rockefeller University Press | en_US |
dc.rights | This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/). | en_US |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/3.0/ | en_US |
dc.title | SUMOylation of DNA topoisomerase IIα regulates histone H3 kinase Haspin and H3 phosphorylation in mitosis | en_US |
dc.type | Article | en_US |
kusw.kuauthor | Yoshida, Makoto M. | |
kusw.kuauthor | Azuma, Yoshiaki | |
kusw.kudepartment | Molecular Biosciences | en_US |
dc.identifier.doi | 10.1083/jcb.201511079 | en_US |
kusw.oaversion | Scholarly/refereed, publisher version | en_US |
kusw.oapolicy | This item meets KU Open Access policy criteria. | en_US |
dc.rights.accessrights | openAccess |
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Except where otherwise noted, this item's license is described as: This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).