dc.contributor.author | Krause, Mary Elizabeth | |
dc.contributor.author | Glass, Amanda M. | |
dc.contributor.author | Jackson, Timothy A. | |
dc.contributor.author | Laurence, Jennifer S. | |
dc.date.accessioned | 2017-06-13T17:00:01Z | |
dc.date.available | 2017-06-13T17:00:01Z | |
dc.date.issued | 2010-01-18 | |
dc.identifier.citation | Krause, M. E., Glass, A. M., Jackson, T. A., & Laurence, J. S. (2010). A novel tripeptide model of nickel superoxide dismutase. Inorganic Chemistry, 49(2), 362–364. http://doi.org/10.1021/ic901828m. | en_US |
dc.identifier.uri | http://hdl.handle.net/1808/24486 | |
dc.description | This document is the Accepted Manuscript version of a Published Work that appeared in final form in the Inorganic Chemistry, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see http://doi.org/10.1021/ic901828m. | |
dc.description.abstract | Nickel superoxide dismutase (Ni-SOD) catalyzes the disproportionation of superoxide to molecular oxygen and hydrogen peroxide, but the overall reaction mechanism has yet to be determined. Peptide-based models of the 2N:2S nickel coordination sphere of Ni-SOD have provided some insight into the mechanism of this enzyme. Here we show that the coordination sphere of Ni-SOD can be mimicked using the tripeptide asparagine-cysteine-cysteine (NCC). NCC binds nickel with extremely high affinity at physiological pH with 2N:2S geometry, as demonstrated by electronic absorption and circular dichroism (CD) data. Like Ni-SOD, Ni-NCC has mixed amine/amide ligation that favors metal-based oxidation over ligand-based oxidation. Electronic absorption, CD, and magnetic CD data (MCD) collected for Ni-NCC are consistent with a diamagnetic Ni(II) center bound in square planar geometry. Ni-NCC is quasi-reversibly oxidized with a midpoint potential of 0.72(2) V (versus Ag/AgCl) and breaks down superoxide in an enzyme-based assay, supporting its potential use as a model for Ni-SOD chemistry. | en_US |
dc.publisher | American Chemical Society | en_US |
dc.title | A novel tripeptide model of nickel superoxide dismutase | en_US |
dc.type | Article | en_US |
kusw.kuauthor | Krause, Mary E. | |
kusw.kuauthor | Glass, Amanda M. | |
kusw.kuauthor | Jackson, Timothy A. | |
kusw.kuauthor | Laurence, Jennifer S. | |
kusw.kudepartment | Chemistry | en_US |
dc.identifier.doi | 10.1021/ic901828m | en_US |
kusw.oaversion | Scholarly/refereed, author accepted manuscript | en_US |
kusw.oapolicy | This item meets KU Open Access policy criteria. | en_US |
dc.identifier.pmid | PMC2810650 | en_US |
dc.rights.accessrights | openAccess | |